在更新世，陆桥的反复形成和消失对种群遗传结构已产生持久的影响。海南岛是国际生物多样性热点地区之一，岛屿种群持续了多个冰川周期，我们预计其隔离和接触的交替周期已驱动了种群和物种的分歧。本项目将以大陆和海南岛共有的大青脊竹蝗、黑翅竹蝗和贺氏竹蝗为对象，拟应用统计谱系地理学方法，结合线粒体和核基因序列以及微卫星数据，研究更新世气候变化对这3种竹蝗的种群遗传结构和动态历史的影响。我们重建竹蝗谱系发生关系，比较其谱系地理格局，估算其有效种群大小及不同竹蝗谱系间的分歧时间，检测其种群分歧时间与气候事件发生时间的一致性；结合生态位建模技术，推断竹蝗的冰期避难所，进而探讨这些竹蝗不同地理种群现代分布格局的历史成因和演化过程。本项目对于揭示气候对生物的作用规律以及生物对气候变化的响应有着重要的科学价值，对于今后研究大陆性岛屿物种多样化具有重要意义，同时也可为我国林业竹蝗灾害的预测提供一定的科学依据。

The repeated formation and loss of land-bridges during the Pleistocene have had lasting impacts on population genetic structure. Hainan Island in China is one of the world’s biodiversity hotspots, where island populations persisted through multiple glacial cycles; alternating periods of isolation and contact are expected to have driven population and species divergence. Here, using statistical phylogeographic methods, we combine mitochondrial and nuclear sequence data with microsatellites to dissect the impact of Pleistocene climate change on population genetic structure and demographic history in these bamboo locusts Ceracris nigricornis laeta, C. fasciata and C. hoffmanni common to the mainland and Hainan Island in China. Phylogenetic relationships among the bamboo locust lineages are reconstructed, the loucst population diversity and geographical pattern are compared, and divergence times among the different bamboo locust lineages are calculated, as well as the population differences and climate events time consistency are tested. Combined with ecological niche modeling technology, the glacial refugia of bamboo locusts are inferenced, and then historical causes and evolution process forming the geographic pattern of modern distribution are explored in the different populations of the three species of bamboo locusts. The project has important scientific value for revealing the law of the role of climate on organisms and the response for organisms to the climate change, and is of great significance for the study of continental island species diversification, and also can provide certain scientific grounds for the forecast of bamboo locust disasters of forestry in China.