miR159在花发育以及盐害和ABA响应方面起重要作用，但对miR159是否调节植物低磷胁迫响应及机制还不清楚。我们前期研究发现低磷抑制大豆miR159e-3p在根部表达，其靶基因是一个受低磷诱导的编码MYB类转录调控因子的基因, 表明miR159e-3p可能调节大豆低磷胁迫响应。. 我们以大豆为实验材料，通过分子生物学和遗传学等研究手段，开展如下研究：（1）定量PCR和融合报告基因方法确定miR159e-3p和靶基因的时空表达模式，特别是对低磷胁迫的响应模式；（2）过表达miR159e-3p和靶基因对大豆响应低磷胁迫的影响；（3）过表达大豆MIM159e-3p以及突变靶基因进一步研究其调节低磷胁迫响应的机理；（4）确定靶基因的转录因子活性和通过ChIP-seq技术在基因组水平上确定靶基因调控的下游基因。解析miR15e-3p调节大豆应答低磷胁迫响应的分子和生理机制。

Mi159s play important roles in flower development, and the responses to salt stress and ABA. But the roles of miR159s in response to phosphate (Pi) deficiency and underlying mechanisms remain elusive. We previously found that soybean miR159e-3p, one member of miR159 family, was repressed in roots under low Pi conditions, and target of which was transcription factor Myb-encoding gene, which was induced by Pi deficiency，indicating that miR159e-3p appear to regulates soybean’s responses to low Pi. . In this study, we employ soybean to carry out the following studies at physiology, molecular biology, and genetics levels: (1) to determine the expression patterns of miR159e-3p and its target gene through quantitative real-time PCR and GUS-fusion approaches, especially their responses to Pi starvation; (2) to explore the role of miR159e-3p and its target gene in P signaling via overexpression of them in soybean; (3) to decipher the functions of miR159e-3p and its target gene through overexpression of MIM159e-3p (miR159e-3p mimicry)and the mutated target gene (miR159e-3p resistant);(4) to determine the transcription factor activity of the target gene and the binding sites of target gene in genome-wide level through ChIP-seq techniques. We aim to reveal the molecular mechanisms of soybean's responses to low Pi regulated by miR159e-3p, and provide new clues for improving phosphorus nutrition efficiency in soybean in future.