针对山东省肾综合征出血热（HFRS）发病率居高不下、疫区范围不断扩大、时有暴发流行、疾病负担重的形势，课题组自2012年开始收集HFRS病人和宿主动物标本及周围环境资料，发现在疫源地类型、病毒型别、进化等方面出现一些新特征，并且发现了汉坦病毒（HV）新的亚型，这可能与该病持续高发，区域暴发流行，疫区时空演变规律息息相关。本研究在此基础上，继续收集相关标本与资料，综合利用现场流行病学、分子生物学、生态学、地理信息系统技术与方法，旨在阐明山东省HFRS疫情特征及其时空演变规律；确定主要流行HV的种类、基因型别和亚型以及与国内外主要流行毒株的进化关系；探讨不同型别HV迁移变化特征及疫源地的时空演化规律；进而构建山东省HFRS发病风险生态位预测预警模型，进行HFRS疫源地划分和区域风险评估，为提出针对性的预防控制策略和措施提供理论依据，并为全国HFRS预防控制推广提供科学参考。

Hemorrhagic fever with renal syndrome (HFRS) is still a serious public health issue with high incidence, extending endemic areas and heavy disease burden in Shandong Province. Our previous studies have collectted data since 2012, and found the type of the natural foci, genotype and subgenotype of hantavirus（HV） were different compare with previous reports This study is proposed to carry out a field investigation on HV in several representative areas in Shandong. On the basis of collection of patients and animal host samples and environment information, molecular epidemiological, molecular biological, ecological and geographic information related methods and technologies will be used to clarify the distribution characteristics and geographic variation of HFRS. The genotype and subgenotype of HVs in different areas in Shandong Province and evolutionary relationship of hantaviruses in different genealogies will be illuminated. The evolutionary rate and the evolutionary regularity of HVs will be analyzed. Regular pattern of spatio-temporal dynamics processes of HVs diffusion and the risk factors for infection will be elucidated. Furthermore, we will establish a risk prediction model of HFRS to identify and evaluate the natural foci using ecological niche model combined with animal host habitat characteristics. These results will provide scientific basis for formulating prevention and control measures of HFRS. The ultimate goal is to provide the theoretical basis for control of the diseases caused by HVs.