活血化瘀中药治疗冠心病血瘀证的科学实质是中药现代化研究的核心内容之一。我们前期研究发现，血瘀证的病理变化与肾上腺素β2受体-G蛋白调控的效应器官生理功能密切相关，而活血化瘀中药的常见配伍方式-川芎丹参配伍具有潜在的协同调控β2受体-G蛋白信号通路的作用。鉴此，本课题拟通过Bioactivity based UPLC-Q/TOF筛选平台，实现对川芎及丹参β2受体活性成分的快速鉴定，并以Chou-Talalay中效原理为依据，借助血栓形成关键受体抗凝作用的三重定量分析明确配伍配比关系。利用冠心病血瘀证模型、Vegfr2-luc转基因鼠及心肌、平滑肌细胞，应用GPCR高内涵表型分析、活体成像、高通量PCR芯片等方法，围绕β2受体-G蛋白信号通路，阐明协同增效的分子作用机理，构建川芎丹参相须配伍治疗冠心病血瘀证的成分-靶点-通路多维网络，诠释基于β2受体-G蛋白通路的活血行气配伍的现代生物学基础。

How to heal the mechanism of blood circulation promoting with scientific language, is one of the core content of modern Chinese medicine. Our previous studies proved that the role of β2-adrenergic receptor activation mechanisms and signaling pathways related to blood circulation herbs. Meanwhile, the combination of Rhizoma Chuanxiong and Radix Salvia miltiorrhiza might show a synergy effect on β2-G protein pathway. Therefore, through the Bioactivity based UPLC-Q/TOF screening platform, to the aim of realize the efficacy of Chuanxiong and Salvia miltiorrhiza’s ingredients rapid identification. By Chou-Talalay combination index equation and phenotypic analysis of key receptor on platelet, to investigate the compatibility and matching principle of Chuanxiong and Salvia miltiorrhiza. By the blood stasis model, Vegfr2-luc transgenic mice, myocytes and smooth muscle cells, with the use of modern research methods included GPCR phenotype analysis, fluorescein tag living imaging technology and high-throughput real-time PCR chips, researches will be carried out around β2AR-G protein signaling pathway to clarify the material basis of its efficacy and molecular mechanism of action. While taking advantage of the classic prescription of blood circulation, we also try to investigate the integration of compatibility studies related signaling pathways regulating β2 receptor and to construct a ingredient-target-pathways network of coordinated regulation of Chuanxiong and Salvia miltiorrhiza. Based on the clarify of the biological basis of prescriptions of blood circulation, the scientific connotation on regulation of β2 adrenergic receptor – G protein pathway activation of blood circulation promoting and Qi activating treatment will be discovered.