花香挥发物对植物种群的繁衍与外源伤害的防御具有重要作用，而对于人类来说，花香挥发物不仅改善花卉品质，还能用作香精提取与化妆品加工的原料。蜡梅是我国著名的香花植物，花开冬季，深受人们喜爱。前期研究表明，蜡梅花香挥发物主要为β-罗勒烯、芳樟醇、乙酸苄酯和苯甲醇等成分，而新近发现的无香品系‘WS001’缺乏后三个组分。本项目以无香与有香品系为材料，建立转录组—蛋白组—代谢组数据库，通过不同品系间的差异比较，从基因转录—蛋白合成到代谢产物三个层面分析，了解花香代谢途径关键底物以及花香挥发有机物（VOCs）的积累、消耗以及途径支路竞争到最后花香产物形成的调控机制，确定主要挥发产物合成的酶基因，并进一步对候选基因进行功能验证与时空表达分析，通过构建文库筛选与其互作的转录因子，对之进行功能鉴定与调控研究，从而明确蜡梅花香最终产物合成的分子调控网络，为揭示蜡梅花香挥发物合成机理及花香分子育种提供依据。

Floral volatiles of plants are very useful for propagation and extension of their population and protection of themselves from injury. Whereas from mankind opinion, floral fragrance can improve the quality of flowers, and can be used as materials of essential oil extraction and cosmetics industry. Chimonanthus praecox is a famous plant and loved by Chinese people because of its fragrant flowers blooming in winter. The studies showed that the floral volatiles of the plant were mainly controlled by β-ocimene, linalool, benzyl acetate, benzylalcohol etc.. A new clone with flowers of no fragrant odor was recently discovered and the last three components didn’t exist in it. In this project, the transcriptome, proteome and metabonome of flowers of the two clones respectively with and without fragrant odor were sequenced and established, and their databases were then compared between two clones. On the basis of their differences between the two clones, we can speculate the mechanism that the substrate and the floral volatile organic compounds (VOCs) accumulate, are consumed and divided into different paths, and finally are synthesized to final floral volatils. Then the key synthase genes encoding the floral volatiles were determined, and their qPCR expression and function were studied furthermore. The transcription factors (TF) involved in regulating the key synthase genes were identified using library construction. Then the function and interaction among the TFs and the genes were studied at last. Finally the molecular networks regulating the biosynthesis of final floral volatile were probed out from the above studies. It will play a foundation for that finally revealling the biosynthesis mechanism of VOCs and breeding the new flower with pleasing floral volatiles using molecular manipulation.