归肝经理论是祖国医学药性理论的重要组成部分，但其生物学本质尚不清楚。现代研究表明归肝经中药通过雌激素样作用参与神经—内分泌—受体网络，调控肝—肾—脑生理功能的多个环节，为此我们在符合中医“整体观”原则的基础上，提出“归肝经与靶器官雌激素/受体（ER）相关联”的假说。前期工作已证实经典归肝经方剂逍遥丸的雌激素样作用，本项目将其用于典型肝郁证模型，以“方剂-活性组分-靶器官（ER）-差异性生物标示物”为主线，通过多组分药代动力学表征逍遥丸全成分的体内分布，检测靶器官ERα、ERβ表达，评价肝主藏血、主疏泄功能主要药效学指标；在此基础上，利用代谢组学表征逍遥丸作用前后的差异代谢物，构建其效应代谢通路网络并挖掘其生理意义，全面解释归肝经的生物学本质。本研究将初步阐释中药归肝经的科学内涵，为方剂归经理论研究提供新思路。

As a key part of Traditional Chinese Medicine meridian theory, the biological basis of liver meridian is still unclear. Our earlier multiterm studys found that the function of liver – kidney - brain system are related to the key target - estrogen receptor (ER). Thus, a new study of liver meridian basis based on metabolism and ER networks regular was proposed in this foundation, which was conformed to the basic theory of Traditional Chinese Medicine. In this reseach, the essence of liver meridian of traditional formular XiaoYao Pills with perimenopausal liver-qi stagnation syndrome rats are evaluated by metabolism, multiple components pharmacokinetic study, main function of liver function, and expression of ERα and ERβ in liver-kidney-brain system. The project will provide technical support and experimental accumulation for exploring biological basis of liver meridian theory of XiaoYao Pills.