骨质疏松症合并OA模型与单纯OA动物模型相比，前者对于关节软骨的损害明显高于后者，因此认为骨质疏松症可能是OA发病的一种危险因素或加速膝骨关节炎病情发展。此外，随着OA的进展会引起骨量减少，诱发骨质疏松症的出现。本研究拟从以下两个方面展开：. (一)分别利用软骨下骨骨量增加小鼠模型、去卵巢模型（OVX）、OA模型、去卵巢复合OA模型分别研究软骨下骨骨量增加和减少对膝骨关节炎退变的作用，从而证明骨质疏松与膝骨关节炎发生发展的作用。. (二)观察补肾填精方通过抑制OVX小鼠骨丢失而延缓膝骨关节炎退变的药效作用，从而进一步阐明中医“肾主骨”理论和异病同治的科学内涵。

Osteoporotic animal model that accompany with osteoarthritis (OA) always causes significant impairment on articular cartilage in contrast to OA.These findings indicate that osteoporosis may be one risk of the occurrence or at least accelerate the process of OA. In addition, the risk of osteoporosis will be more pronounced as the OA go worsen. Our investigations will be performed to address the following two points:. 1. Our study first want establish that osteoporosis play its roles in promoting the development of OA. We selected the mice models as: 1) high bone mass in subchondral bone, 2) OVX- induced bone loss, 3) OVX with OA, to investigate the regulatory effects of the changes of bone mass in subchondral bone in affecting the development of OA.. 2. We try to address the scientific contents of the theory of“Kidney dominating bone”in TCM by observing the protective effects of TKNEP in attenuating degeneration of articular cartilage in knee that induced by OVX.