植物病害严重影响农作物的产量和品质，选育和推广抗病品种是防控作物病害最经济、有效和环保的策略。然而病原菌小种快速适应性变异往往导致专化抗性的丧失，因此全面深入了解植物与病原菌的互作以及寄主抗性机制对开发新的病害防控策略显得尤为迫切。水稻是世界上最重要的粮食作物之一，稻瘟病是水稻生产过程中最重要的真菌性病害。本项目以课题组前期工作中克隆的广谱抗稻瘟病基因Piz-t以及其相应稻瘟菌效应蛋白AvrPiz-t为基础，研究稻瘟菌效应蛋白AvrPiz-t靶标蛋白APIP5介导的水稻细胞死亡和抗病性功能，结合APIP5的转录活性和亚细胞定位调控分析，揭示转录因子APIP5在水稻-稻瘟菌互作中的调控机制，阐明AvrPiz-t-APIP5-Piz-t介导的水稻抗稻瘟菌防御反应机制，为建立新的农作物防控策略提供思路。

Plant diseases seriously affect crop yield and quality. The most effective and environmentally friendly approach to control plant diseases is the use of resistant cultivars. However, because of the instability in pathogen populations, most resistant cultivars often become susceptible within a few years of release. A thorough understanding of plant-pathogen interactions and host defense responses to diverse pathogens is essential for developing new approaches to control plant diseases. Rice is one of the world's important food crops and rice blast, caused by the fungal pathogen Magnaporthe oryzae, is the most serious rice fungal diseases. We previously cloned the broad-spectrum R gene Piz-t in rice and its cognate Avr gene AvrPiz-t in M. oryzae. In this project, we will analyze the function of the transcription factor APIP5, a target of the effector AvrPiz-t, in the control of cell death and disease resistance and determine APIP5 transcription activity and subcellular localization. We aim to establish the rice blast resistance regulation model mediated by AvrPiz-t-APIP5-Piz-t. The proposed project will provide new insights into blast resistance mechanism and new approaches to control plant diseases.