课题组前期对黑龙江省盐碱地引种栽植的沙枣苗木研究发现，丛枝菌根化苗木能够在含盐量1.2%的土地上良好生长，表现出很强的耐盐特性。本项研究利用菌根学、细胞结构学、分子生物学及植物生理学等相关知识，对丛枝菌根化沙枣苗木在不同浓度盐胁迫下的响应机制开展研究。主要研究内容包括：沙枣苗木根际丛枝菌根真菌对盐胁迫响应的行为特征；利用透射电镜分析不同处理苗木根系细胞的超微结构；应用电子探针结合X射线分析仪分析钠离子在根细胞微域的分布特征；采用高通量测序技术及生物信息学和分子生物学方法解析沙枣菌根共生体的耐盐基因；通过对苗木生长生理、光合生理、渗透平衡、抗氧化防御等相关指标的测定，评价菌根化沙枣苗木对盐胁迫的生理响应机制。项目预期成果可深入挖掘优异的耐盐基因资源，进一步完善菌根化沙枣苗木的耐盐理论，对菌根化沙枣苗木在盐碱地上的推广应用奠定理论基础，为松嫩平原盐碱地生态治理提供技术支撑，具有重要的现实意义。

Our previous research of introducing Elaeagnus seedlings in saline-alkali soil of Heilongjiang Province found that arbuscular mycorrhizal seedlings can grow normally in the soil with salt content of 1.2%, which manifested strong salt-tolerance characteristics. We conducted research about response mechanism of arbuscular mycorrhizal Elaeagnus seedlings under different concentrations of salt stress depended on mycorrhizology, the science of cell structure, molecular biology, plant physiology and other related knowledge. The main contents of research include: behavioral characteristics response of Elaeagnus seedlings rhizosphere arbuscular mycorrhizal fungi to salt stress; ultrastructure analysis of different treatment seedling root cells with transmission electron microscopy; analysis of sodium ions distribution characteristics of micro-domains in the root cells with electron probe and X-ray analyzer; Elaeagnus mycorrhizal salt-tolerance genes seeking through high throughput sequencing technologies, bioinformatics and molecular biology methods; evaluation of physiological response mechanism of mycorrhizal Elaeagnus seedlings under salt stress by measuring physiological growth, photosynthetic physiology, osmotic balance, antioxidant defense and other related indicators. The expected results of the project can deeply dig the gene resources of salt-tolerance, further improve the salt-tolerance theory of mycorrhizal Elaeagnus seedlings. This study can provide theoretical basis of mycorrhizal Elaeagnus seedlings application in saline-alkali soil and technical support for ecological management in saline-alkali soil of Songnen Plain, which has important practical significance.