中文摘要
竹子具有独特的开花现象,其经历了漫长营养生长后的成化转变机理是植物界一直关注却仍悬而未决的难题,由于其开花周期长达几十年上百年,长期以来缺乏完善的研究成花转变的实验体系,导致目前竹子开花的研究积累非常薄弱。本项目拟从转录组和表观遗传学角度,基于申请人团队长期以来所积累和构建的麻竹自然开花体系以及乌脚绿竹组培诱导开花体系,对开花前后的不同阶段组织器官开展研究,以期发现与竹子成花转变相关的基因及其调控网络,初步阐明竹子成花转变的遗传调控模式。本项目的研究对于开启探索竹子开花这一独特的植物发育现象具有重要的理论意义,同时,本项目的研究可以发现预示竹子开花的分子标记,在竹子开花死亡前进行竹林更新,从而促进竹林的可持续生产,因此亦具有重要的应用价值。
英文摘要
The flowering of Bamboos is an unique phenomenon. The mechanism of bamboo flowering transition after very lengthy vegetative growth has long been a focus of botanists, which is still an unsolved mystery. Since their flowering interval are up to tens or hundreds years, elegant experimental systems for studying bamboo flowering transition are still lacking, resulting in poor understanding of the flowering mechanism in bamboos. Based on the natural flowering system of Dendrocalamus latiflorus and the tissue culture-induced flowering system of Bambusa edulis, this project will explore the transcriptomics and epigenetics of flowering transition by comparing the tissues of different phases before and after flowering. Our aim is to elucidate the genetic regulation of bamboo flowering transition. The project is of important scientific significance for exploring the unique flowering mechanism of bamboos. Meanwhile, the molecular markers indicating bamboo flowering that will be identified in this project could be used in bamboo cultivation to avoid the losses from bamboo flowering and death afterwards. Therefore, the project is of value in bamboo forestry.
