橡胶是关系国民经济及国防建设的重要战略物资，由胶孢炭疽引起的橡胶树炭疽病是影响橡胶产量的重要病害。P型ATP酶家族基因与植物病原真菌的致病性密切相关，但该家族基因在植物病原炭疽菌中的鉴定与功能分析未见报道。本项目拟在胶孢炭疽菌全基因组序列获得及验证其一个钙调P型ATP酶基因参与致病功能，以及胶孢炭疽菌P型ATP酶基因家族分析基础上，采用基因敲除/互补技术逐一观察和分析ATP酶基因敲除突变体与致病性的关系，鉴定出影响致病力和生长发育的P型ATP酶基因；并对其中与致病相关的基因进行功能验证，分析其蛋白亚细胞定位和时空表达模式，解析蛋白序列中保守结构域和保守位点的功能。通过研究，对橡胶树炭疽菌P型ATP酶家族基因进行鉴定，并明确其在致病机制中的作用，这不仅有助于进一步阐明植物病原真菌P型ATP酶家族基因的致病机理，也为作物炭疽病的抗病育种策略的制定提供理论依据。

Rubber trees (Hevea brasiliensis) are perennial crops that takes important place in our national economie and defense goods, it also brings excellent welfare to local small farmers. Colletotrichum gloeosporioides(Penz) Sac. is one of the major causes of declining yields of rubber tree in most rubber plantations.Despite the fact that C.gloeosporioides is ubiquitous pathogen, little is known about the fungal molecular characters that are important for pathogenicity. P-type ATPases are ubiquitous, and they are important enzymes that participate in cation trans-membrane transport, cell signal transduction, and stability of the cell membrane. Published evidence suggests that a great lot family members of P-type ATPases were looked up, but the molucular identities of these enzymes have not yet been revealed. However, the biological function of the P-type ATPases of Colletotrichum species is still little known.. In previous study, we identified a Ca2+ P-type ATPase gene which was important to pathogenicity of C.gloeosporioides by loss of gene function method. In this progress, we will analyze P-type ATPases in three Colletotrichum species for which complete genome sequence data are available,and identify the pathogenicity related genes of them by the gene-deletion assays and pathogenicity tests. After that, gene complementary assays will be performed to further confirm their functions. We also will investigate the gene expression profiles and protein subcellular localization to known when and where they function. Additionally, we will try to find out conserved sites in their peptide sequences, and create amino acid substitutions to analyze their contributions to functions of gene. After completing the research mentioned above, the members of P-type ATPase family of three Colletotrichum species will be analyzed, and roles of the genes of P-type ATPase in pathogenesis of C.gloeosporioides be elucidated. These would be contributed to develop a new effective control of colletotrichum diseases of crop.