长期连作棉田黄萎病日益严重直接影响新疆棉花产业健康发展。研究表明，土传病害发生与连作作物根际土壤化感物质累积、微生物群落变化等密切相关。生物炭和生物有机肥都具有调节土壤微生态，吸附和降解土壤化感物质，减缓病害发生等作用；同时生物炭能促生微生物。由此申请人认为棉秆炭配施生物有机肥可能是一种有效减轻连作棉花黄萎病的新方法。本研究以施用棉杆炭、生物有机肥及二者配施条件下连作棉花为研究对象，通过提取棉花根际土壤化感物质，采用高压液相色谱等测定化感物质成分，分析变化规律；采用传统培养和分子生物学方法分析土壤微生物多样性；同时监测棉花黄萎病病菌变化、测定棉花体内防御性保护酶等；分析阐明棉秆炭配施生物有机肥对连作棉花根际土壤化感物质和微生物多样性及植株防御性保护酶的关系，明确棉秆炭配施生物有机肥对棉花黄萎病发生的影响。研究成果将为防治棉花黄萎病等土传病害提供理论依据。

The increasingly serious Verticillium wilt disease caused by Long-term continuous cropping had directly affected to healthy development of XinJiang cotton industry.Recent research showed that soil-borne disease was closely related with soil ecological environment,especially accumulation of allelochemicals in rhizosphere soil and change of microbial community.Cotton stalk and bio-organic fertilizer could adjustment of soil microbial ecology,adsorption and degradation allelochemicals and inhibit pathogen growth in rhizosphere soil.At the same time biochar can promote microbial.So the applicant think that cotton stalk with bio-organic fertilizer will be probably a new method to reduce Verticillium Wilt disease.In this project,continuous cropping cotton will be taken as the object of this study.We will focus on the effects of cotton stalk with bio-organic fertilizer on allelochemicals of continuous cropping cotton rhizosphere soil,microbial diversity and protective enzyme activity in cotton by determining substances and variation of allelochemicals,using soil microbial ecology and statistical methods.At the same time,we will definite to effect of cotton stalk with bio-organic fertilizer on Verticillium wilt disease.The results of these studies will provide theoretical basis and practical value for controlling cotton soil-borne diseases by cotton stalk with bio-organic fertilizer.