铝胁迫下植物根尖胼胝质积累是重要的铝毒害机制。课题组前期研究发现，β-1,3-葡聚糖酶基因（SbGlu1）通过调控甜高粱胼胝质降解而提高其抗铝性，且耐铝和铝敏感品种SbGlu1的转录水平差异是由于启动子序列存在差异所致。因此，本研究欲通过（1）寻找SbGlu1的调控基因、蛋白；（2）克隆分析与抗铝性相关的转录因子SbSTOP1并寻找SbSTOP1的互作蛋白以及下游调控基因；（3）解析SbGLU1及SbSTOP1与互作因子之间的调控机制等3个方面的研究，以图进一步阐明Al诱导甜高粱根尖胼胝质积累的调控途径，为深入解析甜高粱抗铝及铝毒害机理提供科学支撑。

Aluminum (Al)-induced callose accumulation is one of Al toxicity mechanism in higher plants. Our previous study has found that over expression of SbGlu1 encodingβ-1,3-glucanase enhance the Al tolerance in arabidopsis by regulating the the decomposition of callose. The transitional difference of SbGlu1 between the Al tolerance and sensitive sweet sorghum can contribute to their variance in the sequence of promoter. Thus, present study included following contents (1) Investigating the upstream regulatory transcription factor and interacting protein of SbGLU1 by yeast one/ two hybrid library screening. (2)Functional analysis was performed on the transcription factor SbSTOP1, which putatively related closely to Al resistance in sweet sorghum. The regulatory downstream genes were selected by chromatin immune co precipitation and yeast two hybrid library selection. (3) Functional analysis was performed on the selected transcription factors or interactive genes by over-expression in the rice and rice art1 mutant. With above research, it is expected to elucidate the regulatory pathway of Al-induced callose accumulation mechanism in sweet sorghum.