青蒿素是治疗疟疾基本药物,需求量巨大，降低生产成本、提高产量是当前研究热点。黄花蒿（Artemisia annua L．本文统称青蒿）腺毛是青蒿素合成、分泌、积累及储存的场所，其正常发育、密度及合成效率决定了青蒿素产量，而腺毛发育及其与生物合成途径的关联研究基础非常薄弱，已成为通过生物技术提高青蒿素含量的瓶颈。申请人长期从事青蒿素合成及腺毛发育研究，通过转录组测序，构建互作网络等筛选出一系列和腺毛发育及青蒿素合成相关的基因，其中MYB家族的MYB0、MYB23、MYB106在腺毛发育及青蒿素合成中起到了重要调控作用。本项目通过CRISPR/Cas9敲除MYB基因并进行一系列生物学实验，揭示MYB体内功能。通过ChIP-Seq、EMSA、Y2H、CO-IP等揭示受MYB调控的基因，构建调控网络，解析青蒿素合成及腺毛发育的分子机制，为提高青蒿素产量，保证青蒿药材的应用与可持续发展提供理论支撑。

Artemisnin is the basic medicine for malaria treatment, the huge quantity demands now make it urgent to decrease cost and increase production. The glandular trichome of Artemisia annua L. is the very part where the artemisinin is active in synthesizing, secreting, accumulating and storing. The production of artemisinin is determined by the normal development of glandular trichomes, density, and synthetic efficiency. The combined research between glandular trichomes development and its biosynthesis passway has become the bottleneck for improving artemisnin production. The applicant has worked on the artemisinin synthesis and development of glandular trichomes for more than five years, screened series of genes related to the development of glandular trichomes and the synthesis of artemisnin. MYB0, MYB23 and MYB106 gene among the MYB family plays significant role in regulating the development of glandular trichomes and the synthesis of artemisnin. In this subject, the MYB gene will be knocked down or knocked out by using CRISPR/Cas9 system to research its function in vivo. To find out the genes and regulating nets, which are regulated by MYB through ChIP-Seq，EMSA, Y2H，CO-IP et al. To analysis the molecular mechanism of the artermisinin synthesis and the development of glandular trichomes. Finally, to improve the production of Artemisia annua, provide theoretical support for the application of artemisinin - derived medicine and its sustainable development.