从免疫耐受角度来研究变应性鼻炎（AR）是目前的研究热点。Foxp3+treg是调节免疫耐受的主要细胞，具有抑制多种炎性细胞，减轻症状作用。Foxp3是其关键的转录因子，受Foxp3基因调控。近来表观遗传学发现Foxp3基因的CpG位点甲基化修饰可调控Foxp3+treg，但具体机制尚未明确。中医药治疗AR有独特之处且疗效确切。益气温阳方（YWF）是治疗AR常用方剂。课题组最新研究表明YWF可上调Foxp3+treg，诱导免疫耐受；但是否通过对Foxp3基因甲基化调控来完成，目前尚不清楚。预实验显示：YWF可增加Foxp3mRNA和Foxp3表达,并且增加Foxp3+Treg数量。且本方中的部分成分被证实可影响基因甲基化，因此本课题设想通过临床样本、细胞培养和动物实验三个层次来研究YWF是否通过Foxp3甲基化调控Foxp3+Treg。这将为中药复方治疗AR机制研究提供理论依据。

To study the allergic rhinitis from the perspective of immune tolerance (AR) is a research hotspot at present. Foxp3+treg is the main cell regulation of immune tolerance, inhibit inflammatory cells, relieve the clinical symptoms. Foxp3 is the key transcription factor, regulation by Foxp3 gene. Recently, epigenetics found Foxp3 gene CpG sites methylation can regulate Foxp3+treg cell, but the specific mechanism is not yet clear. Chinese medicine treatment of AR has unique and curative effect. Yiqi Wenyang Fang (YWF) is commonly used in the treatment of AR prescription. The latest research group showed that YWF can upregulate Foxp3+treg cell induced immune tolerance; but whether the methylation status of Foxp3 gene promoter to complete, it is not clear. Preliminary experiments showed that YWF can increase the expression of the Foxp3mRNA and Foxp3, and increase the number of Foxp3+Treg. And the middle part of the composition of proved to affect gene methylation, so this topic idea through three levels of clinical samples, cell culture and animal experiments to study whether YWF via Foxp3 methylation regulation of Foxp3+Treg. This will provide a theoretical basis for the study on the mechanism of traditional Chinese medicine in the treatment of AR.