循环肿瘤细胞（CTCs）与癌症转移密切相关，其具有很大的异质性，真正能形成癌症转移的仅占很少一部分，对CTCs进行功能分析（分泌蛋白与胞内蛋白同时检测），找出具有高度转移潜能的CTCs，这对肿瘤诊断具有重要意义。但由于CTCs数目极少（1ml血液只有几个至几十个），对检测提出较高的要求，基于微流芯片的单细胞蛋白分析凭借其独特优势，成为CTCs功能分析的发展趋势。本项目拟在前期工作基础上，构建基于集成式微流芯片的CTCs单细胞功能分析系统,包括:（1）利用CTCs物理特性（表面微观结构、介电特性）与生物学特性（表面抗原），完成CTCs的高效捕获；（2）通过泵阀控制与高密度抗体复合物阵列，实现CTCs单细胞分泌蛋白与胞内蛋白的同时检测;（3）利用该系统对早期非小细胞肺癌患者术后的化疗进行评估及复发监测。本系统的建立将为肿瘤诊断技术的发展做出贡献，为癌症侵袭转移机理研究提供更加有效的技术平台。

Circulating tumor cells (CTCs) are closely related to cancer metastasis. CTCs have great heterogeneity and only a small part of CTCs have metastatic potential. So to count all CTCs number is not enough. To find the high metastatic potential CTCs and explore the mechanism of cancer metastasis, we should detect each CTC’s function (secreted proteins and intracellular proteins simultaneously detection). For such low number of rare cells (only several to tens in 1ml blood), it is hard to detect at single cell level. It is a trend to use microfluidic chip for CTCs’ single cell functional analysis. Based on previous work，this project is planned to build an integrated microfluidic system for single cell functional analysis of CTCs. This system is composed by three parts: (1) Recapture the CTCs by using the cell’s physical characteristics (surface microstructures, dielectric properties) and biological characteristics (cell surface antigen);(2) Simultaneously detect CTCs’ secreted proteins and intracellular protein by using PDMS valves and high density antibody compound array; (3) Using such system to analysis CTCs from early non-small-cell lung cancer (NSCLC) patients after surgery, we plan to assess chemotherapy and monitor recurrence. In summary, the establishment of such system will contribute to the development of cancer diagnostic technology and provide a more effective platform for cancer metastasis research.