香草兰为多年生热带特色香料作物，广泛应用于食品、化妆品等行业，具有“天然食品香料之王”的誉称，在我国海南、云南等地区有广泛种植。连续多年种植，微生物区系失衡，长势变差，土传病害根茎腐病频发，出现连作生物障碍，制约了香草兰产业的健康发展。前期研究表明施用微生物有机肥可降低根茎腐病发病率，减轻连作生物障碍，但施用微生物有机肥后土壤微生物群落结构特征和土壤理化性质有何变化？本项目拟通过连续施用微生物有机肥调控导病型土壤微生物环境，并阐明调控机制，为消减香草兰连作生物障碍提供理论依据，同时对消减其它多年生热带作物连作生物障碍也具有重要的借鉴意义。

Vanilla (Vanilla planifolia Andrews) is a rare tropical spice crops used in the agro-food industry. It has earned the reputation of “king of natural flavours”. The planting area of vanilla in China is mainly distributed in Hainan and Yunnan province. Vanilla is a perennial plant, the continuous cropping disturbed soil microflora balance, resulted in yield decline and soil-borne plant pathogen increase. Our previous researches have found that application of bio-organic fertilizer could effectively suppress the disease, and reduce the successive cropping obstacles. However, the characteristic of soil microflora created after bio-organic fertilizer application in rhizosphere soil need investigation. In the present research, we are aimed to regulation of rhizosphere microflora, soil physical and chemical properties in disease suppressive fields of vanilla by application of bio-organic fertilizer and its formation mechanisms will be studied. This research can provide a scientific theoretical basis for preventing the successive cropping obstacles of vanilla planting. Moreover, it has important reference meaning for other perennial tropical crops.