超声引导肿瘤消融是一种最为常用的引导方式。但二维超声存在：肿瘤浸润边界和微癌栓难以准确判断；缺乏依据肿瘤边界制定的精确引导措施和监控设备；消融的安全边缘未能精确定量测量等局限。因此，通过基础研究探索可精确判断肿瘤边界和微癌栓、实时精准肿瘤消融计划及可立体量化安全边缘的评估系统，对提高肿瘤局部治疗疗效及病人长期生存率具有重要意义。高分辨三维超声造影及弹性成像、Fly-Thru对显示肿瘤病灶边界浸润和微癌栓情况及评估实时观测治疗有一定优势。因此，本研究拟通过体内、外实验利用以上新技术确立评估肿瘤边界侵袭度和检测微癌栓的方法，并在已建立三维超声造影术前术后的实时非刚体配准基础上，通过三维数据采集，图像分割配准及融合等基础研究，研发三维超声虚拟规划-评估平台，并利用该平台进行实时立体手术引导、监测及评估，以解决目前二维超声诊疗的不足，提高消融治疗疗效，对推进我国超声引导下肿瘤微创治疗具有重要意义。

Ultrasound-guided tumor ablation is one of the most commonly used image-guided modes. 2D ultrasound has some limitations in clinical application as follows: the difficulty to determine the tumor infiltrating edge and micro tumor thrombus, the lack of accurately guiding measure and monitor equipment based on the tumor boundary; It is difficult to determine quantitatively the ablation safe edge. Therefore, through the basic research to explore the evaluation system which can judge the tumor boundary accurately and micro tumor thrombus, carry out the real-time, feasible and precise tumor ablation plan and evaluate the stereo quantitative safety margin, it is important to improve the effect of local therapy for tumor, and the long-term survival of patients. High resolution 3D CEUS, elastography and Fly-Thru have certain advantages in showing the infiltration boundary of the tumor lesions and micro tumor thrombus, and evaluating the real-time treatment. Therefore, this study intends to through the experiment in vivo and in vitro and using the above new technology to establish the method to evaluate the invasion degree of the tumor boundary and detect the micro tumor thrombus, and on the basis of establishing 3D CEUS pre- and post- treatment by the real-time non-rigid registration in the past, through the basic research about the 3D data acquisition, image segmentation, registration and fusion, to develop the 3D ultrasound virtual planning-evaluation platform. Using this platform to guide, monitor and evaluate the real-time stereo operation, helps to solve the problem of 2D ultrasound diagnosis and treatment at present, to improve the effect of the ablation, which is important for promoting the development of ultrasound-guided minimally invasive tumor therapy in China.