以往研究发现通过单一cDNA文库筛选寄生虫有效抗原基因，缺乏结构性功能表位和无法实现高表达等特性，使筛选基因过程处于mRNA转录水平。希望捕获标识性抗原基因对寄生虫实时产生的免疫抗体难以实现，致使对筛选所获得的抗原基因仍然停留在实验的初级研究阶段，实际应用于寄生虫病临床诊断和免疫预防分子的研制受阻。在前期工作基础之上，本研究拟以猪带绦虫/囊尾蚴为实验研究对象，采用噬菌体肽库筛选技术和双向电泳印迹筛选技术，对虫体不同发育时期抗原基因进行筛选，以期筛选出标识性和保护性强的靶标抗原；利用分子生物学、细胞及免疫学技术与方法鉴定期特异、高敏感及强保护性的抗原基因；为建立适用于人猪带绦虫/囊尾蚴病免疫学诊断和猪囊尾蚴病的临床检测的研究奠定基础，渴望揭示该虫体在不同的发育阶段抗原表达的消长变化规律及抗原在虫体感染过程中的作用机制。同时为类似的食源性寄生虫病，尤其是为寄生虫免疫性抗原的研究提供新的思路。

The reported diagnosis antigen genes acquired by cDNA library of the previous studies were lake of the structural epitope and low expression levels which make the gene only transcription to mRNA without expression. absented the capture capability and the antibody arrest ability in immunity during parasites infection. And the absence impeded the genes application in clinical diagnosis, assay and vaccine study. On the basis of preliminary work, by using the phage peptide library triage technique and dielectrophoresis technology, the study will focus on the antigen genes of different developmental stages of Taeniasis/cysticercosis ,and filter the identification and protection epitope to overcome the absence. The isolated target antigen gene will be charactered through molecular, cell biology and immunology technology. The result of the study will successful contribute to vaccine exploit and clinical diagnosis of Taeniasis /cysticercosis. It will reveal antigen expression variance and molecular biology mechanism in different life phase of Taeniasis/cysticercosis, and it is also significant reference to study on immunity antigen of foodborn parasitosis in the species.