利用同源模建和分子对接技术研究新酶的空间构型及其活性中心，研究酶活性中心与底物的表面扩散、吸附及产物从酶制剂表面解离过程，研究磷脂酶酶解产物中Sn-1位上官能团空间结构，羟基氧孤对电子的特性，使其有利于生成Sn-1,3-DAG异构体，研究使产物中的FFA有利于植物甾醇酯生物合成的机理，并探讨催化顺序对各功能产物的关联特性；对纳米磁性固定化酶（磁酶）结构表征及修饰，研究消除底物对假性失活磁酶活性中心的空间位阻，研究对失活磁酶进行定向攻击切断C-N键并重新接枝修饰和载酶的机理；探讨间歇条件下磁酶多效定向催化的工艺条件，模拟磁酶在磁流化床中达到稳定的散式流化态时的操作条件；将纳米磁酶限定在三相磁流化床内，使毛油中的磷脂连续、分阶段、分层次与特性磁酶多效定向催化并分离，提高磁酶的选择性，为传统脱胶、脱酸工序转化为功能性脂类的生物合成过程提供理论支撑。

Spatial configuration and the active center of new enzyme will be researched using the technologies of homology modeling and molecular docking, and the active center of enzyme and the surface diffusion and adsorption of molecular substrate and the dissociation process of product from the enzyme surface will be investigated. The spatial structure of functional group on sn-1 position in enzymatic hydrolysis product of phospholipase and characteristics of hydroxyl oxygen lone pair will be researched，it is conducive to transform of sn-1,3-DAG isomers，and it will be researched that the mechanism of FFA in the product is propitious to the biosynthesis of phytosterol ester, and the association characteristics of catalytic sequence of each function product will be investigated. The structure of nano-magnetic immobilized enzyme will be characterized and modified, and steric hindrance of substrate on active center of false inactivation of magnetic enzyme will be eliminated, it will be researched that inactivated magnetic enzyme is oriented attacked in order to cut the amide C-N key and graft modification and enzyme immobilization.The process conditions of multi-effect oriented catalysis of magnetic enzyme in bacth conditions will be discussed, and operating conditions of stable and distributed fluidization state of magnetic enzyme in magnetic fluidized bed will be simulated. Nano-magnetic enzyme will be restricted in three-phase magnetic fluidized bed, and the phospholipid in crude oil will be catalyzed by multi-effect oriented using specified magnetic enzyme and separated in continuous, stages and levels, the selective of magnetic enzyme will be improved, it will provide theoretical support for changing the traditional processes of degumming and deacidification into process of functional lipids.