针刺是治疗脑卒中后肌痉挛的常用有效手段，但是其中枢作用机制仍不明确。现代医学认为痉挛是因高级中枢抑制性信号减弱或消失导致的下位中枢运动神经元兴奋，γ-氨基丁酸（GABA）和甘氨酸（Glycine，Gly）是中枢神经系统内最重要的两种抑制性神经递质，因此它们有可能是参与针刺治疗脑卒中后肌痉挛的重要分子。我们前期研究证实脑干和脊髓中的GABA参与了针刺治疗局灶性脑缺血模型大鼠肌痉挛的过程，但是Gly是否参与以上过程以及Gly与GABA在该过程的作用地位及相互关系尚不明确。基于此，本课题提出“Gly和GABA共同参与了针刺对高级中枢的抑制作用，且两者存在协同作用”的科学假说，拟以调整阴阳的中医理论为指导，局灶性脑缺血大鼠模型为载体，观察针刺治疗脑卒中后肌痉挛的疗效，并运用分子生物等技术探讨Gly是否参与针刺的中枢抑制作用及其与GABA之间的作用关系，为针刺治疗脑卒中后肌痉挛提供新的理论依据。

Acupuncture is commonly used in the treatment of post-stroke spasticity, but the central mechanism remains unclear. Modern medicine suggests spasm is due to the lower central neurons excited caused by the advanced nerve central inhibitory signals weaken or disappear. GABA and Gly are two of the most important inhibitory neurotransmitter in the central nervous system. Our previous studies have confirmed the GABA in brain stem and spinal cord paticepate in the procesee of acupuncture treatment for spasticity of focal cerebral ischemia model rat. But whether Gly participates in the above process and the mutual relationship with GABA is unclear. Thus, we put forward the hypothesis that Gly and GABA both paticipate in the proceess of acupuncture treatment for advanced nerve central inhibitory effect and there is synergistic effect existed. We aim to observe the therapeutic effect of acupuncture for muscle spasm after stroke base in focal cerebral ischemia model rats based on the theory of adjusting Yin and Yang of Traditional Chinese Medicine, and explore whether Gly participates in the the central inhibition effect process of acupuncture and the relationship with GABA by using molecular biology and immunohistochemical techniques for providing new theoretical basis on acupuncture treatment for post-stroke spasticity.