本项目针对竹材木质素、木质素-碳水化合物复合物（LCC）含量高的组成特点，围绕破解制约竹材纤维素糖化木质素的物理屏障和LCC的化学屏障，研究碱性预处理主要破坏制约纤维素糖化的物理屏障、阿拉伯糖苷酶降解LCC中木聚糖与木质素连接的糖苷键破坏制约纤维素糖化的化学屏障的反应机理。通过竹材碱性预处理对纤维素糖化关联因子的影响、竹材半纤维素一级结构信息分析和LCC结构解析、木质素和LCC在预处理过程中的行为与机理、LCC在纤维素糖化过程中的行为与机理、竹材碱性预处理和基于阿拉伯糖苷酶辅助水解耦合破解竹材纤维素糖化的物理屏障和化学屏障、基于阿拉伯糖苷酶协同水解竹材纤维素的机理与动力学的研究，阐明木质素、LCC抗竹材纤维素糖化的作用与机理，揭示木质素、LCC在竹材碱性预处理和纤维素糖化过程中的迁移规律、结构变化与反应机理。研究结果可为竹材纤维素糖基平台技术方法的建立提供理论依据。

According to the composition characteristics of bamboo containing higher content of lignin and lignin carbohydrate complex (LCC), for the purpose of breaking physical and chemical barriers restricting bamboo cellulose saccharification, the mechanism of breaking physical barriers restricting bamboo cellulose saccharification by alkaline pretreatment and breaking chemical barriers restricting bamboo cellulose saccharification by glycosidase hydrolysis linked between xylan and lignin with arabinosidase were investigated. Through a series of research containing effects of alkaline pretreatment on correlation factors of cellulose saccharification, the primary structure analysis of bamboo hemicellulose and the LCC structure analysis, the change and mechanism of lignin and LCC in the process of alkaline pretreatment, the change and mechanism of LCC in the process of cellulose saccharification, the integrated process to break the physical and chemical barriers restricting bamboo cellulose saccharification by alkaline pretreatment and arabinosidase assisted hydrolysis. The purpose of this project will reveal the inhibition of lignin and LCC on bamboo cellulose saccharification, and the removal, structure change and reaction mechanism of lignin and LCC in the processes of alkaline pretreatment and cellulose saccharification. The results can provide basis for establishment of efficient sugar platform technology from bamboo.