组蛋白乙酰化修饰是真核生物表观遗传学调控的重要方式之一。组蛋白去乙酰化酶 (HDACs) 介导乙酰化修饰在植物生长发育及基因表达调控中发挥着重要作用。然而，HDACs对果实成熟与衰老生物学过程的调控机制至今仍不清楚。我们前期的研究发现，番茄HDAC家族成员SlHDA1和SlHDA3在破色期或成熟期的果实中表达水平很高，均定位于细胞核中，说明它们在果实成熟衰老中发挥着重要调控作用。本项目拟通过转基因技术、组织切片和生理生化方法研究番茄SlHDA1、SlHDA3对果实成熟衰老的影响，同时分析组蛋白乙酰化修饰情况。利用RNA-seq测序、酵母双杂交和双分子荧光互补技术筛选和鉴定SlHDA1、SlHDA3所调控的靶基因，并结合染色质免疫沉淀技术分析其表观遗传学调控方式。研究结果不仅可以揭示组蛋白乙酰化修饰对果实成熟和衰老的调控机制，也可为通过表观遗传调控果实成熟和耐贮性提供理论基础。

Histone acetylation is an integral component of the epigenetic network in many eukaryotes. Histone deacetylases (HDACs)-medicated acetylation plays key roles in plant growth and development and gene regulation. However, less known about the regulatory mechanism of histone acetylation in fruit ripening and senescence. Our previous study found that HDAC family gene SlHDA1, SlHDA3 have high level of expression during tomato ripening. In this project, the involvement of SlHDA1 or SlHDA3 genes in tomato fruit ripening and senescence will be investigated by transgenic method. The modification of histone acetylation will be analyzed in transgenic plants. The target genes of SlHDA1 or SlHDA3 will be determined by transcriptomic analysis, yeast two-hybrid and bimolecular fluorescence complementation (BiFC). In addition, the epigenetic regulation of SlHDA1 or SlHDA3 will be confirmed by ChIP assays. The results will illuminate the molecular mechanism of epigenetic regulation in fruit ripening and senescence by SlHDA1 and SlHDA3. Also, our study will provide new clues for regulating fruit ripening and storage property by epigenetics.