心脏干细胞移植促进心肌细胞再生、修复受损心肌，为心肌梗死开创了新的治疗前景。目前促进心肌细胞再生的治疗靶标，正逐渐从诱导心脏干细胞分化，向心脏干细胞促进心肌细胞分裂再生转移。基于课题组前期研究及文献报道，鹿红方既可通过增强心脏干细胞对心肌细胞的旁分泌作用，也可能通过增强心脏干细胞对心肌细胞的直接接触作用，促进心肌细胞再生，而PI3K/Akt通路为心脏干细胞促进心肌细胞分裂再生的下游信号通路。根据以上内容提出：鹿红方可通过增强心脏干细胞对心肌细胞的直接接触作用和旁分泌作用，激活心肌细胞PI3K/Akt通路，促进心肌细胞分裂再生。本研究拟制备特殊标记心肌细胞的小鼠心梗模型、心脏干细胞-心肌细胞穿透小室联合培养模型，探讨鹿红方激活心脏干细胞促进心肌细胞再生的机制，为中医药辅助干细胞疗法促进心肌再生提供依据和思路。

Cardiac stem cells transplantation can promote the cardiomyocytes regeneration and repair damaged cardiac muscle,thus creates a new outlook for the treatment of myocardial infarction.Currently the therapeutic target of promoting cardiomyocytes regeneration is gradually moving from inducing cardiac stem cells to differentiate into cardiomyocytes to promoting the division and proliferation of cardiomyocytes by cardiac stem cells.Based on our early study and the literature reports,Luhong formula not only can promote cardiomyocytes regeneration by enhancing the paracrine effect of cardiac stem cells to cardiomyocytes,but may also by enhancing the cell-cell contact effect of cardiac stem cells to cardiomyocytes.At the same time, cardiac stem cells can promote cardiomyocytes divide and proliferate through activiating the PI3K/Akt signaling pathway.Hence we proposed that Luhong formula can promote cardiomyocytes regeneration based on enhancing the cell-cell contact effect and the paracrine effect of cardiac stem cells to cardiomyocytes by activating the PI3K/Akt signaling pathway in the damaged cardiomyocytes after myocardial infarction.In order to test our hypothesis,we created a murine acute myocardial infarction model with a specific marker on cardiomyocytes and co-cultured cardiac stem cells and cardiomyocytes in a transwell contact-free system.This study can provide evidence and strategy for using Chinese medicine to promoting cardiomyocytes regeneration.