课题组在上一个基金(NO:30973801)研究中发现,艾灸治疗血管性痴呆(VD)存在着促脑内血管新生机制,这与新近研究Neurovascular Niche(神经血管小生境)理论高度吻合,然艾灸的神经-血管互生促长的胞外蛋白机制不明,成为课题组下一步研究的目标。本研究用增强绿色荧光蛋白(EGFP)标记神经干细胞(NSCs),并与内皮祖细胞(EPCs)共培养,构建"小生境"共植体,移植于VD模型大鼠脑内.艾灸治疗,免疫荧光双标激光共聚焦追踪NSCs的分化、成熟过程特异蛋白表达及其与新生微血管的关系；围绕EGFP阳性神经元,在激光共聚焦显微镜下计数和定位NSCs和EPCs分泌可相互作用的胞外信号蛋白,分析协同关系,探明艾灸调控促神经新生胞外信号蛋白cross-talk机制,研究艾灸治疗VD的作用途径和规律,为艾灸治该病提供依据。"神经血管小生境"理论和EGFP追踪技术在针灸研究领域未见报道。

In the last Fund (NO:30973801) the research group found moxibustion in treatment of vascular dementia (VD) existing the mechanism of promoting angiogenesis in the brain, it is highly consistent with Neurovascular Niche theory which was reported recently. However reciprocal extracellular proteins mechanism of the neuro-vascular alternate promoting is unknown,and become a research target in the next step. Neurovascular Niche was reported recently as a vascular bed microenvironment of the adult brain which the neurogenesis dependent on highly and become a focus of nerve injury reparation researching. in this study,the VD model will be established . the NSCs (neural stem cells) marked with EGFP (enhanced green fluorescent protein)will be applied, co-cultured with the EPCs (endothelial progenitor cells) in vitro to Construct co-implant of Neurovascular niche, and transplanted in VD model rat brain via lateral ventricle.treating of moxibustion on head acupoints and setting control groups. immunofluorescence double labeling and confocal laser microscope will be utilized to track specific protein expression in differentiation, maturation of the NSCs process.the extracellular signaling protein secrete from NSCs or EPCs will be detected synchronously and to be analyzed of cooperative relationship . on the above to ascertain the mechanism of moxibustion regulation of neurogenesis. all of these to research the pathways and mechanisms of moxibustion.the purpose is to provid basis date for the disease treatment by moxibustion. neurovascular niche theory and EGFP tracking technology in acupuncture study field has not been reported. regularity and mechanism .