甘蓝枯萎病是一种毁灭性的土传病害,其中1 号生理小种（Foc1）是危害我国甘蓝生产的优势病原菌。鉴定Foc1的毒性及非毒性效应子，并分析其调控寄主免疫反应的分子机制对于解析甘蓝枯萎病菌的致病机理具有重要意义。本研究拟通过结合基因组、重测序、转录组、蛋白组等组学方法和分子遗传实验对Foc1的效应子进行鉴定。在此基础上，通过构建GFP 融合蛋白对目标基因进行亚细胞定位、构建目的基因的删除片段鉴定其功能域、通过免疫共沉淀(Co-IP)鉴定效应子的互作蛋白等方法系统解析效应子的功能及其调控寄主免疫反应的分子机制。该研究对于丰富尖孢镰刀菌的致病机理具有重要的意义，并可为甘蓝枯萎病控制及育种策略提供理论支持。

Fusarium oxysporum f. sp conglutinans (Foc), a major soil-bornefungal pathogen, is one of the most destructive diseases of cabbage worldwide. Foc race 1(Foc1) has spread over major cabbage producing areas in north China, causing huge losses to cabbage industry. Identification of the effectors of Foc1 and analysis of their molecular mechanism in immune suppression are significant important to understand the molecular mechanisms for Foc1 pathogenicity. In this study, we plan to identify the pathogenic effectors of Foc1 by combining the omics methods, including genomics, resequencing, transcriptomics and proteomics, with molecular genetic experiments. The candidate effectors will subject to stringent validation experimentally, including gene deletion, complement, and hypersensitive responses experiment. We will further analyze the function of the effectors through a series of molecular experiments, such as subcellular localization of the effectors, constructing the deletions and identification of targets genes for the effectors by co-immunoprecipitation. Our goal is to identify pathogenic effectors for Foc1 and elucidate the molecular mechanism of immune regulation, and the results will provide theoretical guidance for establishing innovative control methods and breeding strategy of resistant cabbages.