慢阻肺患者长期咳嗽咳痰可增加急性加重（AECOPD）和死亡风险，纤毛功能障碍、粘液高分泌和细菌负荷的恶性循环是主要原因。项目组提出COPD稳定期存在本虚标实，治疗需整合调控、精准扶正，补肺颗粒可改善症状且减少急性加重，与整合调控粘液高分泌、细菌负荷及炎症有关，但无法阐明其精准扶正机制。纤毛自噬与气道防御功能受损相关，细胞自噬为揭示中医气虚痰瘀提供思路。项目将通过在香烟烟熏大鼠气管内注入肺炎链球菌建立AECOPD模型，基于纤毛自噬探讨中药单纯扶正和扶正祛邪及西药祛痰/抗氧化对细菌感染后COPD稳定期动物模型的“扶正”机制。以正常人体和COPD患者气道上皮细胞为研究对象，采用气-液面共培养、CCK8、MDC和实时定量PCR等方法，观察补肺颗粒对气道上皮细胞自噬的影响和机制。从整体和细胞水平研究补肺颗粒的“扶正”机制，促进整合调控、精准扶正作用的科学表达，丰富中医正邪理论，创新COPD病机治法。

COPD patients with long-term recurrent cough and sputum may increase the risk of acute exacerbation (AECOPD) and death. Ciliary dysfunction, hypersecretion of mucus and nausea cycle of bacterial load is the main reason. The project team proposed the existence of healthy qi deficiency and pathogens strong in stable COPD, and its treatment needs integrated regulation / Precision promotion. And Bufei granule can improve symptoms and reduce exacerbations, which related to the integrated regulation of airway mucus hypersecretion and reduction of bacterial load, but it can not clarify its mechanism of Precision promotion. Cilia autophagy is associated with impaired cilia autophagy clearance. The clarification of cell autophagy mechanism provides a new idea for revealing positive qi deficiency and phlegm of TCM. The project will establish AECOPD model by making rats cigarette smoked and injecting Streptococcus pneumoniae in rats tracheal, and discuss the mechanism that how the Chinese medicinal herbs for strengthening healthy Qi, herbs for strengthening healthy Qi and eliminating pathogens, and Western medicine for expectorant / antioxidant strengthen healthy qi of rats with bacterial infection in stable COPD based on cilia autophagy. Airway epithelial cells of Normal human and COPD for the study, the team will observe the effect and mechanism of Bufei granule on the cilia autophagy of lung airway epithelial cells by using gas-liquid culture, CCK8, MDC, quantitative real-time PCR, et al. and explore its internal mechanism. The proiect research "strengthening healthy qi" mechanism of Bufei granule in overall and cellelar level, and expound the nature and content of overall regulatory / Precision promotion scientifically, Which will enrich TCM theory of Healthy Qi and pathogens, and innovate COPD pathogenesis and treatment.