果实早期发育决定了果形和产量，但早期发育调控机理尚不明确。在青年基金实施过程中，研究发现草莓独脚金内酯合成关键基因MAX1转录水平在果实早期发育阶段明显高于其它时期，且随果实发育急剧下降。外施氟啶酮抑制MAX1基因的转录，并延缓草莓果实发育。草莓MAX1基因可以恢复拟南芥独脚金内酯缺失突变体max1-1的多分枝表型，这些结果表明MAX1可能通过合成独脚金内酯调控草莓果实早期发育。鉴于此，项目拟以“红颜”草莓为试材，全面调查MAX1基因与果实内独脚金内酯含量的关系，分析MAX1在草莓果实独脚金内酯合成中的作用；借助“基因功能沉默与过表达”技术，结合时空表达特性明确MAX1调控果实早期发育过程；通过转基因果实转录组和启动子应答元件分析，构建MAX1调控果实早期发育的上下游网络。项目为揭示MAX1基因调控草莓果实早期发育分子机制，并对指导草莓栽培生产和分子改良育种都有十分重要意义。

Early development of fruit determine their shape and yield. However, the molecular regulatory mechanism of early fruit development remains unknown. In previous studies, it was found that the transcriptional level of strawberry MAX1, a key gene in strigolactone biosynthesis, was significantly increased in the early development, but fell dramatically with fruit development. Additionally, fluridone inhibit the transcription of MAX1 gene and delay strawberry fruit development. Strawberry MAX1 may restore the branching phenotype of Arabidopsis strigolactone deletion mutant max1-1, indicating that MAX1 may regulate strawberry early fruit development by strigolactone biosynthesis. Hence, with the aim of analyzing the role of MAX1 in strigolactone biosynthesis, Benihoppe, a cultivar of strawberry, will be used as material to have a complete survey of the relation between MAX1 and the content of strigolactone in fruit. With the RNAi, overexpression, genetic transformation system, and time-space characteristics of the gene, regulation of MAX1 in early fruit development will be clearly elucidated . To build an up- and down-stream regulating network of MAX1 gene in early fruit development, transcriptome of transgenic-fruit and cis-element will be analyzed. Our project is aim at revealing the molecular mechanism of MAX1 in regulating early fruit development and exploring a new hormone network in fruit development of strawberry, thereby contributing to the strawberry molecular breeding with enhanced quality traits.