镉污染已成为食品安全的重要问题之一，微生物法脱镉已成为国际上研究的热点。微生物具有一定的镉抗性和吸附能力是应用该方法的必要前提。本文在前期研究工作的基础上，以具有一定耐盐性的酿酒酵母为研究对象，先研究氯化钠对酿酒酵母镉胁迫时生长特性的影响，再获取该酵母分别在镉胁迫和氯化钠-镉胁迫培养的样品，采用转录组学RNA-seq技术，找出关键的差异显著表达基因，用qRT-PCR验证；采用蛋白质组学同位素标记相对定量技术（i-TRAQ)，找出关键的差异显著表达蛋白，用Western blot验证；联合转录组学和蛋白质组学的研究结果，探寻关键的差异表达基因/蛋白，并进行生物学验证，以期从多层次、多角度解析氯化钠对酿酒酵母镉胁迫的保护作用。本项目的研究成果将为脱镉微生物的改造提供理论依据，丰富微生物脱除重金属的理论研究和应用，指导脱镉技术的实践过程，还为其他耐性微生物的交叉抗性研究与应用提供参考。

As the problem of cadmium pollution in food is becoming more and more serious and the removal of heavy metals via growing microorganisms has received a great deal of attention. On the basis of the previous research, the moderately halotolerant Saccharomyces cerevisiae is chosen as the research object in this study. The growth traits of S. cerevisiae under cadmium stress is studied firstly. Then, S. cerevisiae under cadmium and NaCl-cadmium stress is analyzed by transcriptomics and proteomics. The technology of RNA-seq and i-TRAQ is used in transcriptomics and proteomics respectively, and qRT-PCR and Western blot are used for validation. The combined transcriptomic and proteomics approach is used to explore and verify the differentially expressed genes and proteins, which can parse the protective effect of NaCl on S. cerevisiae under cadmium stress at multi-level and multi-angle. The results of this study can provide theoretical basis for the melioration of cadmium removal microorganisms, enrich the theory of applying growing microorganisms for cadmium removal, instruct the cadmium removal practice and provide references for the research and application of cross-resistance in other tolerant microorganisms.