中文摘要
Q型烟粉虱是当前我国田间主要危害生物型。Q型烟粉虱对绝大多数寄主植物的适应能力通常强于B型烟粉虱。甘蓝是目前已报道少有的有利于B而不利于Q型烟粉虱的寄主植物,其对B和Q型烟粉虱非对称影响机制还不甚清楚。我们采用GC-MS分析甘蓝分别受B和Q型烟粉虱取食为害后诱导产生的挥发性萜类化合物化学成分和含量差异,比较烟粉虱前期取食危害的甘蓝对后续烟粉虱寄主选择、产卵和EPG取食行为的影响。同时,我们采用HPLC和LC-MS分析B和Q型烟粉虱危害后的甘蓝诱导产生的主要芥子油苷化学成分和含量,进一步采用人工饲料方法测定甘蓝受害后诱导产生的主要芥子油苷对B和Q型烟粉虱取食行为、芥子油苷的分泌和主要解毒酶基因表达的差异,从行为、分子和生理角度阐明甘蓝对B和Q型烟粉虱非对称影响及其机制,研究结果可望为Q型烟粉虱的防治提供理论依据。
英文摘要
Whitefly Bemisia tabaci Q biotype is the dominant biotype in wild field in China. It is generally accepted that Q whiteflies are more tolerant to most host plants than B. In contrast, cabbage Brassica oleracea is one of the rare host plants which have been reported to be more suitable to B than to Q, the asymmetric effects and mechanisms of cabbage to B and Q are poorly explored. We use the GC-MS analysis to determine the differences in chemical compositions and contents of volatile terpenoids released from cabbage damaged by B and Q, respectively, and compare the influences of previously damaged cabbage on host selection, oviposition preference and EPG feeding behavior of the subsequent B and Q whiteflies. At the same time, we employ HPLC and LC-MS analysis to investigate the differences in chemical compositions and contents of main induced glucosinolates from cabbage damaged by B and Q, respectively. We further employ artificial diet method to determine the differences of main glucosinolates induced by B and Q on feeding behavior, glucosinolates secretion and the gene expression of main detoxification enzymes between B and Q. From the points of behavior, molecular and physiology, we aim to elucidate the asymmetric effects and mechanisms of cabbage to B and Q whiteflies, which may shed some light on Q whiteflies control.
