铁载体是微生物自身合成的、用于从环境中浓缩和吸收铁营养的一种代谢产物，铁载体在人类铁代谢疾病的治疗和预防、在水产动物疾病防治和环境保护等方面具有重要的用途。但是铁载体合成需要添加外源的稀有L-鸟氨酸。在本项目中，将在高产铁载体Fusigen的酵母菌HN6.2菌株中构建一个能利用葡萄糖直接合成铁载体的新途径，避免添加昂贵的外源L-鸟氨酸。然后利用转录组学和现代基因工程技术研究L-鸟氨酸-N5-羟化酶、NRPSs及其基因和新途径菌株中铁载体合成与调控的关系。所以本项目将使用全新的概念研究酵母菌铁载体的合成和调控，并为酵母菌廉价高效生产铁载体Fusigen提供重要技术基础。

Siderophores are important metabolites synthesized by microorganisms to concentrate and absorb iron from living environments. They also have highly potential applications in medicine and maricultural industries. However, siderophore biosynthesis in yeasts must start from L-ornithine which is an expensive and rare amino acid. In this project, in order to avoid supplementation of L-ornithine in the medium, a new siderophore biosynthesis pathway in the yeast strain HN6.2, which could produce high level of siderophore called Fusigen, will be constructed and the new recombinant strain which has new siderophore pathway can synthesize siderophore from glucose directly. Then, the investigation on relationship between L-ornithine-N5-monooxygenase，NRPSs as well as their genes and siderophore biosynthesis and regulation will be carried out based on transcriptomics and modern gene technology within the new pathway strain. Therefore, in this study, siderophore biosynthesis and regulation in the recombinant strain will be investigated based on the completely new concept and the siderophore fusigen will be directly and efficiently produced from glucose by the newly constructed yeast strain.