番茄是世界上的一种重要蔬菜作物。番茄叶霉病是我国乃至世界番茄生产的主要病害，给番茄生产造成了巨大损失，番茄叶霉病原菌C. Fulvum是生理小种分化很快的一种真菌，目前我国生产上应用的主要是含Cf-5和Cf-9抗病基因的番茄品种，近年来我国部分地区生产上已经出现了含Cf-5和Cf-9抗病基因的番茄品种被侵染的现象。Cf-12抗病基因在反复的抗病性鉴定中对多个生理小种表现出了抗病性，具有很高的潜在应用价值。本项目组在前期的分子标记研究和番茄材料高通量测序数据的研究基础上，拟对Cf-12基因进行精细定位、基因克隆、功能验证、抗病基因专化性演变、抗病反应相关基因的功能以及Cf/Avr互作机理进行研究，并从分子水平上揭示Cf基因的抗病机制，旨在为今后的抗病育种工作提供宝贵的基因资源和理论基础。

The tomato is the world's major vegetable crops.Tomato leaf mold disease, caused by biotrophic fungus C. fulvum, is a serious disease of Solanum lycopersicum (tomato). This disease can reduce both fruit yield and quality, and sometimes even kill the tomato plants. C. fulvum has many physiological races, and new physiological races can develop rapidly , making it difficult to manage this disease. An efficient method to control leaf mold outbreaks is breeding to obtain resistant cultivars. Recently, tomato varieties applicated for resistance breeding mainly contain Cf-5 and Cf-9 resistance genes, but these rasistance genes were over-comed by new pathogen physiologies. The Cf-12 gene, which has been shown to confer resistance to this disease in the field, can serve as a new candidate gene in breeding. Fine mapping, cloning and functional verification of Cf-12 gene, will be carried out based on the previous data from study of gene mapping and tomato material resequencing. And for the purpose of understanding the resistance response mechanism of Cf/Avr interaction, the differentially expressed genes in Cf-12-mediated resistance response will be studied by transcriptome sequencing method. The results will provide us important gene material and theoretical basis for the breeding work in the future.