子宫内膜异位症（EMs）相关疼痛临床治疗困难，发病机制复杂，NGF调控DRG内p38MAPK信号通路，使TRPV1表达增强可能参与EMs疼痛的发病。本课题组前期以活血化瘀，清热利湿法自拟盆痛灵灌肠，临床对EMs疼痛患者效果明显，并可显著提高小鼠痛阈，降低NGF水平。基于以上两点，我们提出“盆痛灵方可能通过NGF-p38MAPK信号通路，影响TRPV1表达，抑制内异症疼痛”的假说。拟通过建立SD大鼠EMs模型,鞘内注射NGF、p38MAPK抑制剂；不同剂量盆痛灵灌肠，观察模型大鼠行为学及内异灶结构变化；另盆痛灵含药血清干预离体DRG细胞，Western blot、PCR法检测NGF/p-p38MAPK/TRPV1等基因及蛋白的表达，研究NGF-p38MAPK-TRPV1信号通路在EMs疼痛中的作用，探讨盆痛灵方通过该通路逆转EMs疼痛的分子机制，为中医药治疗EMs疼痛提供新的、确切的科学依据

Pain is a main symptom of Endometriosis, It is seriously and a tough problem for clinical treatment.the pathogenesis is also complex. NGF regulate p38MAPK signaling pathway of DRG,TRPV1 expression increased, It may participate in the pain of EMs. We form PenTongLing compound by Activate blood, clear heat and wet. Previous clinical study showed that treating EMs pain with PenTongLing compound has a significant effect, animal experiments showed using this compound by enema can significantly increase the pain domain in mice, reduce the level of NGF. Based on the above two points, We put forward the hypothesis " PenTongLing may through contraversy p38MAPK signaling pathway, affect TRPV1 expression, inhibition the pain of Endometriosis ". we will measure the pain domain of EMs rat model，the expression of related signaling molecules and transduction protein like NGF、p38、TRPV1 will detected by immunohistological labeling and molecular biology, transmission electron microscopy will be used to asses the EMs lesions ultrastructure. At the same time, treat the DRG cell invitro with PenTongLing medicated serum at different times，to explore the process of NGF-p38MAPK-TRPV1 signal pathway in EMs pain,and the role of PenTongLing compound in this signal pathway,in order to provide hard scientific evidence for Chinese medicine prevent and treat pain associated with Endometriosis.