重寄生作用是木霉生物防治的重要机制，对重寄生过程中木霉与病原菌相互作用缺乏足够的认识，限制了对木霉重寄生机制的深入了解。本项目拟采用转录组学技术，研究哈茨木霉（Trichoderma harzianum）LTR-2与两种重要植物病原菌立枯丝核菌（Rhizoctonia solani）和畸雌腐霉（Pythium irregulare）的重寄生互作。通过在重寄生过程的不同阶段，利用RNA-seq技术检测互作双方在转录组水平的表达变化，了解哈茨木霉与病原菌相互应答而最终成功实现重寄生的动态过程。通过比较不同病原菌引起的LTR-2转录组变化差异，了解LTR-2识别寄主的机制及产生的特异反应。获得的转录组学信息以及对部分基因的功能验证，对哈茨木霉LTR-2基因组的功能注释有重要意义。因此，本研究将为阐明哈茨木霉的重寄生机制提供重要信息，同时为指导其在农业生产中的科学应用提供理论基础。

Mycoparasitism is one of the important modes that Trichoderma strains employ to antagonize plant pathogens. The lack of sufficient knowledge about the bilateral interactions between Trichoderma and pathogens has limited the insights into mycoparasitism. In this research, transcriptomics technology will be applied to study the mycoparasitic interactions between Trichoderma harzianum LTR-2 and two important plant pathogens, Rhizoctonia solani and Pythium irregulare. At different stages of mycoparasitism, expression changes at transcriptome level of both sides will be analyzed by RNA-seq. Then the dynamic interaction processes that fulfilled the successful mycoparasitism will be elucidated. Through comparing the transcriptomic differences aroused by different pathogen hosts, the mechanisms and specific reactions involved in host recognition will be understood. Furthermore, the transcriptomics data and gene function studies will provide useful information to annotate LTR-2 genome. Therefore, important knowledge to elucidate T. harzianum mycoparasitism might be expected in this study, which will provide theoretical basis for guiding its application scientifically in agriculture.