腺体过度分泌是变应性鼻炎（Allergic Rhinitis，AR）常见病征，其发病机制可能与NF-κB信号通路下调水通道蛋白5有关；尚未有较好的治疗方法。“培土生金”（健脾益肺）法治疗AR是临床最常用且疗效较好的方法，尤其在止涕通窍方面，但机制不明。本研究拟将112只SD大鼠随机分7组，采用基础致敏和鼻腔激发联合方法建立AR大鼠模型，同时烟熏法复合木瓜蛋白酶雾化吸入法和番泻叶泻下法建立肺脾气虚AR大鼠模型，造模成功后用鼻敏方高剂量、低剂量分别干预2周、4周，干预期间记录大鼠行为评分，干预后处死大鼠取鼻黏膜，HE染色涂片观察鼻黏膜病理，免疫组化和western-blot法检测NF-κB和水通道蛋白5，RT-PCR法检测IL-1β、TNF-α、IL-6和水通道蛋白5，从NF-κB信号通路对AR大鼠鼻黏膜水通道蛋白5表达的影响探讨鼻敏方的干预机制，为推广“培土生金”法中药防治AR提供科学依据。

Excessive gland secretion is common symptom of allergic rhinitis, and its pathogenesis may be related to the NF-κB pathway, which can down-regulate the expression of Aquaporin 5.There is no effective treatment so far. Although the exact mechanism remaining unclear，the method of “Enhancing earth to supplement metal” (aslo called “Enhancing Spleen to supplement Lung”) which is the most common treatment with better efficacy，especially on clearing the nasal discharge and opening the nasal orifice.The 112 SD rats will be randomly divided into 7 groups in this research, the rat model of AR will be established by the basic ovalbumin-sensitization combined with nasal provocation, meanwhile，to establish the model of lung and spleen qi deficiency of rats with AR use fumigation combined with papain aerosol inhalation and senna leaf laxation method. After the models established successfully, Bi Min decoction will use at high dose and low dose for 2 weeks and 4 weeks respectively. The score of behavior of rats will be recorded during the treatment period. These rats will be killed after intervention and their nasal mucosa will been taken.Then immunohistochemistry and Western blot will be performed to investigate the expression levels of NF-κB and aquaporin 5 while RT-PCR will be performed to investigate the expression levels of IL-lβ,TNF-α, IL-6 and in aquaporin 5 rat nasal mucosa. Aiming to provide a scientific basis for the promotion of “Enhancing earth to supplement metal”method of Chinese medicine for prevention and treatment of AR, to explore mechanism of Bi Min decoction base on the influence of NF-κB signaling pathway to the expression of aquaporin-5 in the nasal mucosa of AR rats.