竞争排斥原理认为，两个物种/亚种及以下的分类阶元不能同时或长时间共享同一生态位。针对西花蓟马的温室品系和羽扇豆品系同时侵入我国，空间分布格局、生境类型和寄主谱不清楚的问题，本项目采用mtDNA COI和rDNA 28S双基因标记技术以及生殖生物学、种群生态学、种群遗传学和生态适应性模拟的技术与方法，首先研究确定西花蓟马的温室品系和羽扇豆品系是否具有生殖隔离现象，建立品系特异性检测技术；同时通过室内生境模拟实验，阐明两个品系在不同温湿度组合和不同种群比例的演替规律和优势入侵品系；然后分析探讨两个品系不同地理种群双基因遗传多样性和遗传分化，及其与主要生态因子的相关性；进而，明确两个品系的寄主谱、气候生态位分化和环境适应梯度。研究结果对明确西花蓟马两个品系的空间分布格局及其种群演替机制，制定区域性靶向防控措施具有重要意义，并将有助于深入揭示西花蓟马的生态适应性和种群形成机制，丰富生态位分化理论。

According to the competitive exclusion principle, two species cannot occupy the same niche or cannot share the same niche for a long time. The greenhouse strain and lupine strain of Frankliniella occidentalis invaded China simultaneously. Field investigations indicated that the population ratios of the greenhouse strain to lupine strain differ in different geographic regions. Consequently, the damages caused by this thrips are different. However, the spatial distribution pattern, habitat type and host plant species are still keeping unclear. In the present study, double gene markers, mtDNA COI and rDNA 28S, techniques of reproductive biology and population ecology, population genetics and ecological adaptability simulations will be used. Subsequently, taxonomic status of the greenhouse and lupine strain of this thrips will be clarified, i.e., are they two strains of one species that can mate each other freely or are different cryptic species. The technical systems of SS-PCR for specific detection of the greenhouse strain or lupine strain will be established. The dynamics of competition and succession between both strains will be determined under the conditions of different combinations of temperature and relative humidity as well as under different population ratios of greenhouse strain to lupine strain, and the dominant strain will be defined. A labeling technique based on double genes will be established and the synergic relationships between the genetic diversity and differentiation of both strains and adaptability to ecological environment will be investigated. The differentiations of host plant species, climatic ecological niche and gradient of environmental adaptation of both strains will be analyzed. The results should be significant in clarifying the mechanisms of spatial distribution pattern and population succession of the two strains as well as in development of regional target management measures of the thrips. Furthermore, the results should be useful in revealing the mechanism of ecological adaptation and population formation of this thrips species and in rich the theory of ecological niche differentiation.