目前茶园中盲目的施用大量氮肥，严重影响茶叶的品质，造成资源浪费，生态环境恶化。而关于施氮对茶树生理响应机制、碳与氮代谢调控机制、氮素如何影响茶叶品质尚未阐明。本研究以前期筛选出的氮高效与氮敏感型的茶树幼苗为试验材料，探明不同施氮水平对①茶树叶片光合特性、叶绿素荧光特性、叶片氮素含量、活性氧代谢的影响，阐明不同施氮下限制光合速率的主要因素；②茶树生长、氮素同化关键酶（硝酸还原酶 NR、亚硝酸还原酶 NiR、谷氨酰胺合成酶 GS 和谷氨酸合酶 GOGAT）活力的变化规律及相关关系的影响；③茶树叶片碳代谢关键酶（RuBP 羧化酶、蔗糖磷素合成酶(SPS)、蔗糖合成酶(SS) 和转化酶 (Inv)）的影响；④茶树体内干物质积累的变化规律进行分析，探讨碳、氮代谢关键指标对茶树产质量的影响。旨在明确不同施氮水平对茶树叶片光合作用影响及茶树氮、碳代谢的机制，为茶树高产优质栽培提供科学依据。

At present blind application of a large number of nitrogen fertilizer in tea plantation has not only seriously affected the quality of tea, but also resulted in wasting of resources and environmental degradation. So far as, the mechanisms on nitrogen and carbon response and how nitrogen affects the quality of tea tree under nitrogen-stress have not been studied. In this study, we had screened relatively high and low nitrogen-utility germplasm from the tea cultivars of Fujian province. The following aspects have also investigated: ①Effect of photosynthesis system、chlorophyll fluorescence、nitrogen concent、active oxygen metabolism under different supplying nitrogen were analyzed. ② Effect of tea growth、key enzyme content of nitrogen assimilation(nitrate reductase NR, nitrite reductase NiR, glutamine synthetase GS and glutamate synthase GOGAT) were analyzed. ③Effect of carbon metabolism (RuBP carboxylase, sucrose phosphorus synthase SPS, sucrose synthase SS and invertase Inv) were analyzed. ④Effect of dry matter accumulation and key enzyme of carbon and nitrogen metabolism were analyzed. The main objective of this study was to understand effect of photosynthesis system and metabolic mechanism of carbon and nitrogen. The main objective was providing scientific basis high yield and high quality cultivation of tea plant.