果实衰老和劣变的过程伴随着质膜透性增加和结构变化，质膜微区(membrane microdomain)是调节质膜功能特化的基本单位，而质膜微区蛋白则是细胞与病原菌互作过程中行使相关功能的重要组分。Remorins是植物细胞中最早鉴定的膜微区蛋白，但其在果实抗病性应答过程中的分子细胞学机制还有待研究。本项目拟在前期工作基础上，综合运用基因过表达、CRISPR-Cas9和基因沉默技术、荧光标记和实时追踪等方法，开展如下研究：（1）分析Remorins过表达、基因敲除和RNAi株系中果实抗病性应答反应的变化；（2）阐明Remorins在亚细胞水平上的定位模式、互作因子和细胞内循环途径；（3）探明Remorins响应病原菌侵染后内吞及胞内分选途径的变化，解析其对病原菌侵染响应的时空特异性，揭示质膜微区蛋白Remorins基于膜微区蛋白动态的信号激发机制，为研究果实采后抗病性应答机理提供重要线索。

Ripening, aging and deterioration of fruits are always concurrent with structural variations and increase in permeability of plasma membrane, membrane microdomains are basic units for regulating structural compartmentalization and functional specialization of plasma membrane, while functional proteins resided in membrane microdomains are important components for the interaction between cells and pathogenic microorganisms. Remorins are above all the first definitely identified proteins in membrane microdomain, but the underlying molecular and cytological mechanisms for the responses of fruits mediated by Remorins towards pathogenic microorganisms are still largely unclear. The present study is proposed on the basis of our previous work by combining over-expression, CRISPR-Cas9, RNAi, fluorescence label and real-time tracking and other techniques. Emphases will be placed on the studies in three major aspects: (1) to investigate changes in disease-resistant responses of fruits in overexpression lines, knock-out and RNAi lines of REMORINs; (2) to analyze the variations in subcellular localization pattern and constitutive recycling mechanisms of Remorins; (3) to investigate the variations in endocytic events and intracellular sorting of Remorins in response to pathogen infection, finally reveal the spatio-temporal specificity for the responses to pathogen infection, elucidate the mechanisms for signal priming and transduction of Remorins based on protein dynamics in membrane microdomains, and provide new clues for studying the mechanisms for postharvest disease-resistant responses of fruits.