环境污染已成为导致两栖类种群数量下降的主导因素，两栖类生殖力对其种群数量的稳定有着重要作用。然而，在野外自然环境条件下，稀土金属冶选尾矿库渗漏对其周边区域造成的复合污染如何影响当地两栖类的生殖力和种群动态变化，目前尚不清楚。本项目以花背蟾蜍（Bufo raddei）为研究对象，以内蒙古某稀土金属冶选尾矿库周边受污染的水域湿地和相对无污染的小白河黄河湿地保护区为研究样地。进行野外环境下的实地调查和采样，在实验室内采用组织解剖、年龄结构鉴定、组织电镜观察、理化分析和核酸凝胶电泳等方法，对比分析上述两样地各年龄段的不同性别花背蟾蜍的生殖力及影响因素。结合相关种群动态参数指标，探讨稀土金属选冶尾矿库复合污染对花背蟾蜍生殖力的影响机理。确定其生殖力的改变对花背蟾蜍种群动态变化的影响效应。对当地两栖类种群保护及稀土尾矿库污染防控、监测与评价具有重要意义。

Environmental pollution has become the leading cause of amphibian population declines, while their reproductive capacity plays an important role in the maintenance of population size, so dissection the impact of environmental pollutants to amphibians' reproductive capacity is urgently needed.,However, the influence of rare earth tailings combined pollution on amphibian reproductive capacity as well as population dynamics in natural environment is unclear yet. To address this issue, Bufo raddei, a species widely distributed in the north China, is selected to explore the effect of combined pollution of the rare earth tailings dam on amphibian reproductive capacity as well as population dynamics. A rare earth tailings dam in the Inner Mongolia with higher contamination is chose as the research plot, and Xiaobaihe wetland of Yellow River in Baotou, a relatively unpolluted area is chose as control. Technics such as anatomical structure, age structure assessment, electron microscopy, physicochemical analysis, and DNA gel electrophoresis will be used in this study. The differences on the reproductive capacity and population dynamics of B. raddei between the two areas will be comparatively studied. Through the above research, we will figure out whether the compound pollution around the rare earth tailings dam could have an effect on the reproductive capacity of the B. raddei or not, and demonstrate the possible impact pathways and mechanism.