柴胡-白芍系多个疏肝解郁名方如逍遥散、柴胡疏肝散、四逆散等的基础药物。柴胡辛散，疏肝解郁；白芍酸收，养血柔肝，二者散收相合，构成疏肝解郁、调畅情志类方剂的核心药对，现代研究表明此药对抗抑郁作用确切。然而，该药对配伍抗抑郁及其在疏肝解郁方中核心地位的现代科学内涵尚不清楚。本项目在课题组前期发现柴胡、白芍对逍遥散抗抑郁作用贡献较大的基础上，拟整合代谢组学、药代动力学和网络药理学技术对柴胡、白芍配伍前后体外、内化学成分、药代行为及作用机制进行系统研究，以阐明二者配伍的协同增效机制。在此基础上，以逍遥散为疏肝解郁方代表，将药对回归于复方，验证柴胡-白芍药对在复方抗抑郁作用的核心地位，并阐明该药对在逍遥散抗抑郁中的机制。本项目的完成，不仅可揭示柴胡-白芍散收药对协同抗抑郁的配伍机制，而且可为以柴胡-白芍药对为基础药物的疏肝方配伍规律的阐释提供科学依据，同时，为证实中医临床用药的科学性提供研究思路。

Radix Bupleuri - Radix Paeoniae Alba (Chaihu-Baishao) herb pair are basic herbs of many famous shuganjieyu prescriptions such as Xiaoyaosan, Chaihu-shugan-san, Sinisan. Radix Bupleuri, Xinsan, Shuganjieyu, and Radix Paeoniae Alba, Suanshou, Yangxuerougan, they are central herbs of shuganjieyu and tiaochangqingzhi prescriptions. However, modern scientific connotation of importance of Chaihu- Baishao in prescription and composition rules of Chaihu-Baishao herb pairs are unclear. Our previous study results also show that Radix Bupleuri and Radix Paeoniae Alba play main role in antidepressant effect of Xiaoyaosan. On the basis of previous study, the project combind metabonomics pharmacokinetics, and network technology to study overall impact of components both to each other in vitro and in vivo，influence of metabolic characteristics of the active ingredients each other and the differences of significant metabolites and target regulated by herb pair and single herb in depression animal model rats, which clarify the chemical and pharmacological mechanism of compatibility. On this basis, we select Xiaoyaosan as a representative of shuganjieyu prescription, compare the chemical components and mechanism of herb pair and prescription, combined with the results of the drug compatibility mechanism to clarify the antidepressant mechanism of the herb pair in the Xiaoyaosan. The completion of this project, not only can reveal Chaihu-Baishao compatibility mechanism in antidepressant effect, but also can provide scientific basis for clarifying composition rules of shuganjieyu prescription based on Chaihu–Baishao herb pair, at the same time, which can provide ideas and methods to confirm the scientificity of Clinical medicine of Traditional Chinese Medicine.