肿瘤经规范化治疗后仍存在休眠肿瘤细胞，休眠肿瘤细胞通过外泌体分泌改变周围微环境，形成肿瘤复发和转移的“土壤”。研究发现，黄芪多糖可改善机体内环境，逆转肿瘤细胞免疫逃逸，促进肿瘤细胞休眠，阻止体内隐匿性转移，提高肿瘤患者的远期生存率。本课题拟建立荧光标记的高转移人卵巢癌休眠小鼠模型并给予黄芪多糖干预，通过荧光蛋白活体成像系统实时观测休眠肿瘤细胞状态，并对不同干预措施下肿瘤细胞分泌外泌体进行分析和比较，阐明其与肿瘤血管生成和免疫调节的关系，进而研究黄芪多糖逆转肿瘤细胞免疫逃逸，促进肿瘤细胞休眠，阻止肿瘤复发和转移的外泌体调控机制，为其临床应用及进一步研究促肿瘤休眠药物提供依据。

After treatment with standardization，tumor remains dormant tumor cells, dormant tumor cells by means of exosomes change the surrounding environment and form the "soil" of tumor recurrence and metastasis.The studies found that astragalus polysaccharide can improve body environment, reverse tumor cells in the immune evasion, promote the tumor cells rest, prevent dormant metastases in body and improve the long-term survival of cancer patients.This topic proposed the establishment of a high transfer people ovarian cancer dormancy mice model of fluorescent tags and give astragalus polysaccharides intervention, real-time observation by fluorescent protein in vivo imaging system state of dormant tumor cells, analyse and compare the exosomes secreted by tumor cells under different interventions, expounds its relationship with tumor angiogenesis and immune regulation, and then study the exosome regulatory mechanism of astragalus polysaccharides reverse tumor cell immune escape, promote the dormant tumor cells, prevent tumor recurrence and metastasis ,for its clinical application and further research of promote tumor dormancy drugs provide the basis .

肿瘤经规范化治疗后仍存在休眠肿瘤细胞，一旦休眠肿瘤细胞的休眠状态被打破，那么规范化治疗后的肿瘤就会发生复发和转移。研究发现，黄芪多糖可改善机体内环境，逆转肿瘤细胞免疫逃逸，促进肿瘤细胞处于持续休眠的状态，从而阻止体内隐匿性转移，提高肿瘤患者的远期生存率。本课题拟建立荧光标记的高转移人卵巢癌休眠小鼠模型并给予黄芪多糖干预，通过荧光蛋白活体成像系统实时观测休眠肿瘤细胞状态，并对不同干预措施条件下的裸鼠休眠模型的组织及血清进行分析和比较，从而来探讨黄芪多糖逆转肿瘤细胞免疫逃逸，促进肿瘤细胞处于持续休眠的状态的可能作用机制及肿瘤血管生成和免疫调节的关系，为其临床应用及进一步研究促肿瘤休眠药物提供依据。