使用杀虫剂阻断介体昆虫是防控虫传植物病毒病的主要途径。西花蓟马及其传播的番茄斑萎病毒（TSWV）病在我国的危害呈加剧趋势，西花蓟马对多杀菌素的抗性问题也日益凸显，目前蓟马体内与TSWV互作的蛋白因子及功能尚不明确，抗药性是否影响西花蓟马对TSWV的传播及其分子机制如何是亟待解决的科学问题。本项目以多杀菌素敏感和抗性西花蓟马为试虫，拟采用双向电泳和病毒覆盖技术，筛选西花蓟马体内与TSWV特异结合及具有种群差异的蛋白；通过蛋白质谱鉴定和西花蓟马转录组数据库获得匹配蛋白的核苷酸序列，用RT-PCR及RACE技术克隆不同基因的全长序列；采用RNAi技术结合西花蓟马获毒和传毒效率的比较，确定不同受体蛋白基因的功能和在两品系中的差异。研究结果有助于深入了解TSWV与西花蓟马互作的分子机制，阐明多杀菌素抗性对西花蓟马传毒的影响及分子机制，为抗性西花蓟马和TSWV的防控提供理论基础和新思路。

Insect vector control, through the use of insecticides, plays a key role in the prevention and control of insect-borne viral diseases. In recent years, Frankliniella occidentalis (Pergande) have caused increasing serious damages in China, and the Tomato spotted wilt virus (TSWV) transmitted by F. occidentalis has resulted in great losses in some areas. Spinosyns such as spinosad, are the most effective insecticides for F. occidentalis control. However, resistance to spinosad has become an increasingly prominent problem. Whether resistance in F. occidentalis has impact on its virus transmission, to what extent of the impact and what are the molecular mechanisms for the impact are important scientific problems to be solved. So far, it is not clear the proteins interacted with TSWV in thrips and their functions need to be clarified. In the present project three aspects are to be conducted: 1) To screen the different specific binding proteins with TSWV in spinosad-susceptible and -resistant F. occidentalis using 2-D and virus overlay assay techniques. 2) To identify the proteins through protein mass spectrometry and then retrieve the transcriptomes of -susceptible and -resistant F. occidentalis to obtain the nucleotide sequences of the proteins. To clone the full-length sequences of the genes encoding the proteins by using RT-PCR and RACE. 3) To determine the functions of the genes and their differences in the two strains by using RNA interference technique and comparing the difference in virus acquisition and virus transmission efficiency between the two strains. The results will be helpful in further understanding the molecular mechanisms of the interaction between TSWV and F. occidentalis, to clarify the impact of spinosad resistance on the virus acquisition and virus transmission and the molecular mechanisms of the effect. In addition, the study will be provide theoretic bases and new thought for resistant F. occidentalis control and the IPM of TSWV.