水稻(Oryza sativa L.)是最重要的粮食作物之一，水稻花器官发育的分子遗传机制研究对杂交水稻育种具有重要意义。申请者前期研究发现，水稻ADF (Anther development F-box)基因编码一个典型的F-box蛋白，ADF受一个参与水稻绒毡层细胞程序性死亡(PCD) 进程的关键转录因子TDR直接调控，在水稻花药发育和绒毡层降解过程中发挥重要的作用。然而ADF如何参与到水稻绒毡层细胞PCD进程并最终影响花药发育的精确调控机制目前尚不清楚。本课题拟在已有基础上开展以下工作：（1）阐明ADF基因在水稻绒毡层降解过程中执行的生物学功能；（2）研究ADF蛋白与互作蛋白共同参与泛素介导的蛋白质降解途径的机制；（3）构建ADF与水稻花药绒毡层细胞PCD关键基因的调控网络模型。本研究将为水稻雄性不育的分子机理和调控网络研究提供理论支持, 有望创制新型雄性不育材料供育种应用。

Rice (Oryza sativa L.) is the world's staple food crop, understanding the molecular mechanism of floral organ development is very important for hybrid rice breeding. It has been shown in our previous research that, ADF (Anther development F-box), controls anther development in rice and encodes a typical F-box protein. During rice anther development, ADF is directly regulated by the transcription factor TDR, which is one of the key factors of tapetum Programmed cell death (PCD) in rice. However, it remains uncertain that how ADF regulated the development of tapetum PCD in rice. The main works of this study are as follows: (1) The functional analysis of ADF in rice tapetum degradation. (2) The study on the mechanism of ubiquitin-mediated protein degradation through screen substrate interaction protein with ADF. (3) The establishment of regulatory network of ADF for tapetum PCD in rice. This study will provide more theoretical support for the molecular mechanism of rice male sterility and gene regulatory networks. Also it is expected to create new male sterility materials.