腰椎盘突出症（LDH）是临床上常见的腰部疾患之一，推拿有很好的镇痛效果，但目前镇痛机制尚不明确。研究表明，中枢敏化是LDH疼痛发生和维持的主要机制，而SP/NK-1R通路能够强化脊髓背角中枢敏化作用。我们前期研究表明，推拿干预LDH有很好的临床疗效，并且明显降低LDH模型大鼠背根神经节SP水平。据此，我们推测“推拿对LDH镇痛机制可能是通过SP/NK-1R通路抑制脊髓背角中枢敏化”。本课题拟采用疼痛行为学、蛋白免疫印迹及在体电生理等多学科方法，观察推拿按揉法对LDH大鼠脊髓背角SP/NK-1R信号通路的影响，并且研究按揉法对脊髓背角突触传递效能（LTP）的调控作用，从而证实推拿按揉法对腰椎间盘突出症的镇痛机制是通过调控SP/NK-1R通路信号，抑制大鼠脊髓背角中枢敏化作用。本研究在脊髓背角层面上揭示了推拿干预腰椎间盘突出症的镇痛机制，为推拿治疗腰椎间盘突出症的临床推广应用奠定实验研究基础。

Lumbar disc herniation(LDH) is one of clinical common lumbar disorders,Chinese Tuina of analgesia in lumbar disc herniation has better clinical effect, but the analgesia mechanism is unclear.Studies have shown that central sensitization is the main mechanism of pain in LDH and maintain and SP/NK 1 r pathways can strengthen the spinal dorsal horn of the central sensitization effect.Our previous study,Tuina intervention LDH has better clinical analgesic effect, and can significantly reduce the SP level of LDH model rat dorsal root ganglion.On this basis, we speculate"Tuina of LDH analgesia mechanism may be through the SP/NK1R pathway inhibition of spinal cord dorsal horn central sensitization".This topic proposed multidisciplinary approach,such as pain behavior,western blot and in vivo electrophysiology,observe the Anrou method of Tuina of LDH in the rat spinal cord dorsal horn SP/NK1R signaling pathways, and study with method of the dorsal horn of spinal cord adjustment of synaptic transmission efficiency (LTP).To confirm the Anrou method of Tuina the analgesic mechanism of lumbar disc prolapse is through the inhibition of SP/NK1R channel reduces the rat spinal cord dorsal horn central sensitization effect. This study reveals the analgesic mechanism of Tuina ntervention in lumbar disc herniation, to Tuina in the treatment of lumbar disc herniation clinical application experimental study foundation.