毒死蜱作为替代高毒有机磷农药的主要品种，在我国农业生产中使用量大且范围广，对农产品安全生产带来极大的隐患。利用植物内生菌加速农作物体内残留农药的代谢与降解，有望成为控制农药大量使用带来残留污染风险的新途径。我们前期从水稻植株中分离获得了 5 株具有毒死蜱降解功能的内生细菌，接种水稻初步证明内生细菌可有效加速植株不同部位毒死蜱的消解。本项目拟在此基础上，进一步明确不同内生细菌在稻株内的定殖规律，并解析其参与调控水稻植株内毒死蜱消解的贡献及功能。通过抗利福平结合GFP标记研究内生细菌在稻株内的运动轨迹、分布、微观定位及消长动态，优选定殖方式及条件。通过盆栽实验研究内生细菌定殖对水稻植株内毒死蜱消解动态及路径的影响，比较植物体内主要代谢酶系对内生细菌定殖的响应，明确内生细菌定殖是否参与调控农药代谢酶的活性。研究结果可为农产品农药残留污染控制与治理提供新的思路，为保障农药高效、安全使用提供指导。

Chlorpyrifos, which is one of the main pesticides of alternative high toxic organophosphorus pesticides, is extensively used in agriculture production in China, and pose serious risk to the quality and safety of agro-products. To enhance the degradation of pesticide in plant by endophytic bacteria might be a novel method of pesticide residue control. Previously, we have isolated 5 strains of endophytic bacteria from rice plant. Inoculation of the isolated endophytic bacteria has shown increased chlorpyrifos degradation of different parts of the rice plant and grain. In this study, we aimed to further clarify the colonization of the 5 isolated endophytic bacteria and their roles in the degradation of chlorpyrifos in rice plant. By using anti-rifampicin and GFP label to analyze the distribution, movement, location, and dynamic of the endophytic bacteria in rice plant, and optimize the inoculation methods and conditions. The impact on the degradation dynamic and degradation pathway of chlorpyrifos in rice plant by endophytic bacteria inoculations, and the response of organophosphorus pesticide degradation enzymes to bacteria inoculation, were then further explored. The results are of great theoretical and practical significance for the control and management of pesticide residue in agro-products, and also especially helpful for ensuring high-efficiently and safely application of pesticide in agricultural production.