蛹虫草是食药兼用且可以大规模培养子实体的真菌，具有较高营养价值和滋补活性。光照是其子实体发育和类胡萝卜素产生的关键因子，我们前期通过基因敲除发现光受体CmWC-1是其子实体发育和类胡萝卜素产生必不可少的一种转录因子，但是其调控机制不明。本项目以该转录因子为切入点，通过野生型和Cmwc-1敲除株光照前后及子实体发育不同阶段转录组比较分析，预测蛹虫草光受体CmWC-1调控的基因信息；染色质免疫共沉淀分析界定CmWC-1直接调控的靶基因及其结合的DNA序列特征；选择CmWC-1调控的重要转录因子及子实体发育和类胡萝卜素合成的相关基因，通过基因敲除及转录动态分析验证其功能，结合生物信息学分析，揭示CmWC-1、重要转录因子及子实体发育、类胡萝卜素合成相关基因之间的相互关系，最终阐明CmWC-1在子实体发育及类胡萝卜素产生中的调控机制，为蛹虫草子实体优质栽培和类胡萝卜素高效生产提供理论依据。

Cordyceps militaris (L.) Link is one of edible and medicinal fungi with high nutritional value and tonic activity. Light is the key factor for the fruit body development and carotenoid production. Our previous study has confirmed that blue light acceptor CmWC-1 is a nessssary transcript factor for the fruit body development and carotenoid production by gene disruption in Cordyceps militaris. However, the mechanism is not clear. In the present study, a whole-genome gene expression analysis was performed with the wild-type and the Cmwc-1 deletion mutant grown under dark or light conditions to forecast the target gene of this transcript factor. The direct target gene of CmWC-1 and the binding DNA motif was then identified by chromatin immunoprecipitation assay (Chip). The transcript factors and genes related to fruit body development and carotenoid production were found among the target genes of CmWC-1. The relative expression of these genes were analysised and gene disruption was performed to study the functions. The realtion between these genes, tracnscript factors and CmWC-1 will be showed and the regulation mechanism of CmWC-1 on fruit body development and carotenoid production will be presented. This study will lay a foundation for the study on light reaction mechanisms and the molecular basis of the fruit body formation in this fungus and provide a theoretical basis for the high quality and high yield cultivation of this fungus.