质体反向信号在协调光合作用相关的基因（PhANGs）表达中扮演了重要的角色，对于环境胁迫条件下叶绿体的发育和功能维护也是至关重要的。当植物遭受到诸如强光，干旱，高温，冻害，以及病菌侵害等极端的生物和非生物胁迫时，过氧化氢依赖的质体反向信号途径（Hydrogen Peroxide (H2O2)-dependent Retrograde Signaling (HPRS)）对于维护叶绿体的功能是非常重要的。然而，我们对高等植物的HPRS的作用机理却知之甚少。因此，该项目将以前期筛选到的hprs1突变体为研究素材，构建图位克隆群体，鉴定突变基因，系统的研究突变体的生理表型，并综合运用分子生物学、生物化学和遗传学等研究策略来解析HPRS的分子机理。

Plastid retrograde signaling plays a crucial role in coordinating the expression of photosynthesis-associated nuclear genes (PhANGs), and is essential for chloroplast biogenesis and functional maintenance in response to changing environmental stimuli. Among those characterized plastid signaling pathways, Hydrogen Peroxide (H2O2)-dependent Retrograde Signaling (HPRS) plays an important role in maintaining the function of the chloroplast under adverse extreme abiotic and biotic stresses, such as high light (HL), drought, high temperature (HT) and cold, as well as pathogen invasion. However, we only know very little about the underlying mechanism of HPRS in higher plants. Therefore, this project aims to generate the mapping populations and identify the mutant genes for previously isolated hprs1 mutants, to systematically characterize their physiological phenotypes and to dissect the molecular mechanism of HPRS by combining biomolecular, biochemical, and genetic strategies.