本项目以筛选获得的具有改善肠道菌群结构和拮抗氧化损伤功能的益生菌发酵乳杆菌grx08、干酪乳杆菌grx12、鼠李糖乳杆菌hsryfm 1301及其组合为研究对象，利用高血脂大鼠动物模型，围绕外源益生菌对大鼠肠道拟杆菌和乳杆菌水平与构成的调控及肝细胞脂质氧化损伤拮抗效应相关机制问题，应用基因组学、蛋白质组学和免疫组学相结合的方法，研究益生菌对模型动物血清、肝细胞脂质氧化损伤相关蛋白、基因表达水平的影响，探明肝细胞脂质氧化损伤拮抗效应；结合外源益生菌及其作用的目标菌群对体外肝细胞脂质氧化损伤拮抗的主要调节分子及诱导菌群的研究，以期从肠道目标菌群和细胞、基因及蛋白水平上探索益生菌对肝细胞脂质氧化损伤拮抗效应的作用机制，为益生菌发酵乳等功能食品开发提供理论和技术支持。

The function of improving intestinal flora and antagonism to oxidation damage of Lactobacillus fermentum grx08, Lactobacillus casei grx12 and Lactobacillus rhamnosus hsryfm 1301 and their group has been confirmed by our previous research, and the regulation of Bacteroides and Lactobacillus in intestinal and the effect mechanism of antagonism of lipid oxidation damage to hepatocyte of L. fermentum grx08, L. casei grx12 and L. rhamnosus hsryfm 1301 and their group will be studied by using models of hyperlipidemia rat. The antagonistic effect of lipid oxidation damage to the hepatocyte is to be clarified by the effect of probiotics on serum and the expressing levels of related proteins and genes of lipid oxidation to the hepatocyte of rat models based on genomics, proteomics and immunohistology methods, and then the antagonistic effect mechanism is to be explored by combining with the research of the main regulatory molecules and the induced bacteria of the antagonistic effect of lipid oxidation damage to hepatocyte in vitro by which external probiotics and the target bacteria of probiotics intervene. The aim of this project is to explore the mechanism of probiotics of antagonistic effect of lipid oxidation damage to hepatocyte at cellular, genetic and protein levels and the target bacteria, which will provide theoretical and technical support for fermented milk with probiotics and other functional food development.