主疏泄是肝的主要生理功能之一，通过疏泄全身气机以维持人体正常的生命活动，肝的疏泄太过或不及皆可导致人体生理活动异常。细胞是人体最基本的功能单位，肝主疏泄对生命活动的调节必然在细胞层面体现出来。自噬是细胞通过溶酶体降解内部异常蛋白质、清除受损细胞器等以维持自身内环境稳定及物质与能量代谢平衡的重要机制，细胞自噬能力减退或太过都会导致细胞内环境失衡、功能失常甚至死亡,这与肝的疏泄功能非常相似。据此，我们提出“细胞自噬是‘肝主疏泄’功能在细胞层面的具体表现 ”的假说。本课题在前期研究的基础上，围绕这一工作假说，采用行为学与分子生物学等研究方法，从mTOR这一细胞自噬的主要调节通路观测慢性心理应激对大鼠海马神经细胞自噬功能的影响及调肝治法对其调控作用的相关机制，为发展和丰富中医肝藏象及情志病因理论提供实验依据，同时也为中医药抗应激损伤研究提供新的思路。

To dredge and regulate is one of major function of liver, it refers that the liver ensure the free movement of qi and prevents qi stagnation. It is basement of normal life.it can result body disorder if the free coursing of liver losing. Cell is the basic functional unit of body,so that regulating activity of life of free coursing should be expressing on the level of cell. Autophagy is a phenomena of cell relieving abnormal protein and cytoplasm by cytolysosome, and it is very important to maintain stable interior environment of cell and metabolizing equilibrium of substance and energy in the cell. If autophagy is abnormal, the function of cell will be disordered or death. the mechanism of autophagy is like free coursing of liver. so we suggest the hypothesis: "autophagy is the expression of free coursing of liver on the level of cell". this study will surrounding the hypothesis, on the base of our last study, using the way of molecular biology and behavior medicine. To study the mechanism of the method of soothe the liver effect autophagy of hippcampal neurocyte of chronic psychological stress rat from the mTOR signal way. the study will develop the theory of zangxiang and cause of disease. and afford the evidence for finding new drug to antistress.