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水稻适应旱直播幼苗形态特性的关联定位与候选基因研究

水稻适应旱直播幼苗形态特性的关联定位与候选基因研究
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  • 批准号:31671672
  • 批准年度: 2016年
  • 学科分类:稻类作物遗传育种学(C130501) |
  • 项目负责人:梅捍卫
  • 负责人职称:研究员
  • 依托单位:上海市农业生物基因中心
  • 资助金额:62万元
  • 项目类别:面上项目
  • 研究期限:2017年01月01日 至 2020年12月31日
  • 中文关键词: 幼苗;形态;关联;定位;候选基因
  • 英文关键词:Rice;Ecological characteristics;Gene mapping;Gene cloning;Molecular mechanism

项目摘要

中文摘要

采用抗旱品种和机械化旱直播栽培体系可使稻作大幅度节水,现有品种多按移栽或水直播条件选育,未按旱直播优化特性。幼苗通过中胚轴和胚芽鞘的伸长提高出苗速度和整齐度,是对杂草竞争力和高产群体的基础。中胚轴黑暗伸长,见光后停止,形成幼苗对播深自适应机制。本研究拟综合考虑播种深度、中胚轴长度和胚芽鞘长度及比例,提出适应旱直播的早期幼苗形态性状的优化模式,分析土壤水分不足和低温影响以及补偿性状参数。利用我国水稻微核心种质、抗旱水稻品种和全球水稻品种等自然变异群体,已获得全基因组SNP基因型数据,拟开展中胚轴和胚芽鞘长度及其比例、适度深播条件下出苗率和秧苗素质等性状的关联定位。以节水抗旱稻优良恢复系为受体亲本,利用回交后代及其次级分离群体进行QTL精细定位,同时转育适应旱直播条件的节水抗旱稻新种质。采用中胚轴延长受光抑制过程中差异表达基因分析等手段加快候选基因鉴定和克隆,开展目标基因的功能和调控机制研究。

英文摘要

Irrigation water could be largely saved in rice production after application of drought resistant varieties and mechanized dry direct sowing method. Most present varieties were developed for transplanting or wet direct sowing system without optimizing their characteristics for dry direct sowing. Elongation of both mesocotyl and coleoptile increases the speed and uniformity of seedling emergence of deep-sown seeds that is the basis of competition against weeds and high-yielding population structure. Mesocotyl elongated at the dark that stopped after exposure to light. This characteristics support the self-adaptation of seedlings to sowing depth. This study take comprehensive considerations on sowing depth, final lengths of mesocotyl and coleoptile (and their ratio) to propose a optimized pattern of early seedling morphological characteristics. The influence of low soil water content and low temperature are analyzed to estimate compensation of seedling characteristics. Using natural varied populations like mini- core-collection of Chinese rice germplasm, drought resistant varieties and collection of global rice germplasm and ready-to-use SNP genotypic data, association mapping will be conducted for mesocotyl length, coleoptile length, their ratio, seedling emergence rate and quality after properly deep-sowing. Using backcross progenies and derived segregation populations with elite restorers of water-saving and drought-resistant rice (WDR) as the recurrent parents, QTL fine mapping and development of new WDR materials adapting to dry direct sowing will be conducted. Analysis of differential gene expression during the procedure of light inhibition to mesocotyl elongation will be used to accelerate the identification and cloing of candidate genes. Researches on functions of target genes and the regulation mechanism will be conducted in this study.

评估说明

    国家自然科学基金项目“水稻适应旱直播幼苗形态特性的关联定位与候选基因研究”发布于爱科学iikx,并永久归类于相关科学基金导航中,仅供广大科研工作者查询、学习、选题参考。国科金是根据国家发展科学技术的方针、政策和规划,以及科学技术发展方向,面向全国资助基础研究和应用研究,发挥着促进我国基础研究源头创新的作用。国科金的真正价值在于它能否为科学进步和社会发展带来积极的影响。

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